Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
115 |
4.6% |
| Arg[R] |
121 |
4.9% |
| Asn[N] |
138 |
5.5% |
| Asp[D] |
139 |
5.6% |
| Cys[C] |
19 |
0.8% |
| Gln[Q] |
84 |
3.4% |
| Glu[E] |
173 |
6.9% |
| Gly[G] |
71 |
2.8% |
| His[H] |
55 |
2.2% |
| Ile[I] |
189 |
7.6% |
| Leu[L] |
331 |
13.3% |
| Lys[K] |
179 |
7.2% |
| Met[M] |
52 |
2.1% |
| Phe[F] |
132 |
5.3% |
| Pro[P] |
70 |
2.8% |
| Ser[S] |
232 |
9.3% |
| Thr[T] |
137 |
5.5% |
| Trp[W] |
22 |
0.9% |
| Tyr[Y] |
92 |
3.7% |
| Val[V] |
142 |
5.7% |
| Total Length |
2493 |
|
| Instability index (II) |
42.79 |
| This classifies the protein as unstable |
|
| Carbon |
13009 |
| Hydrogen |
20587 |
| Nitrogen |
3389 |
| Oxygen |
3801 |
| Sulfur |
71 |
| Total number of atoms |
40857 |
| Formula = C13009H20587N3389O3801S71 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
259205 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
258080 M-1 cm-1 |
| assuming all Cys residues are reduced |
258080 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
259205 M-1 cm-1 |
|
|
| Codon Bias |
0.067 |
| Codon Adaptation Index |
0.146 |
| Frequency of Optimal Codons |
0.448 |
| Hydropathicity of Protein |
-0.169 |
| Aromaticity Score |
0.099 |
|
