HTA2/YBL003C Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 19 14.4%
Arg[R] 10 7.6%
Asn[N] 8 6.1%
Asp[D] 3 2.3%
Cys[C] 0 0.0%
Gln[Q] 6 4.5%
Glu[E] 5 3.8%
Gly[G] 14 10.6%
His[H] 3 2.3%
Ile[I] 7 5.3%
Leu[L] 17 12.9%
Lys[K] 11 8.3%
Met[M] 1 0.8%
Phe[F] 1 0.8%
Pro[P] 5 3.8%
Ser[S] 8 6.1%
Thr[T] 5 3.8%
Trp[W] 0 0.0%
Tyr[Y] 3 2.3%
Val[V] 6 4.5%
Total Length 132

Instability index
Instability index (II) 53.03
This classifies the protein as unstable

Atomic composition
Carbon 612
Hydrogen 1031
Nitrogen 193
Oxygen 179
Sulfur 1
Total number of atoms 2016
Formula = C612H1031N193O179S1

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 4470 M-1 cm-1

Aliphatic index
Aliphatic index 98.48

Coding Region Translation Calculations
Codon Bias 0.608
Codon Adaptation Index 0.544
Frequency of Optimal Codons 0.748
Hydropathicity of Protein -0.316
Aromaticity Score 0.030