Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
71 |
4.6% |
| Arg[R] |
25 |
1.6% |
| Asn[N] |
51 |
3.3% |
| Asp[D] |
43 |
2.8% |
| Cys[C] |
16 |
1.0% |
| Gln[Q] |
33 |
2.1% |
| Glu[E] |
85 |
5.5% |
| Gly[G] |
94 |
6.1% |
| His[H] |
5 |
0.3% |
| Ile[I] |
96 |
6.2% |
| Leu[L] |
53 |
3.4% |
| Lys[K] |
19 |
1.2% |
| Met[M] |
29 |
1.9% |
| Phe[F] |
36 |
2.3% |
| Pro[P] |
90 |
5.9% |
| Ser[S] |
200 |
13.0% |
| Thr[T] |
432 |
28.1% |
| Trp[W] |
25 |
1.6% |
| Tyr[Y] |
29 |
1.9% |
| Val[V] |
105 |
6.8% |
| Total Length |
1537 |
|
| Instability index (II) |
30.16 |
| This classifies the protein as stable |
|
| Carbon |
6911 |
| Hydrogen |
10996 |
| Nitrogen |
1750 |
| Oxygen |
2539 |
| Sulfur |
45 |
| Total number of atoms |
22241 |
| Formula = C6911H10996N1750O2539S45 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
181710 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
180710 M-1 cm-1 |
| assuming all Cys residues are reduced |
180710 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
181710 M-1 cm-1 |
|
|
| Codon Bias |
0.384 |
| Codon Adaptation Index |
0.259 |
| Frequency of Optimal Codons |
0.647 |
| Hydropathicity of Protein |
-0.163 |
| Aromaticity Score |
0.059 |
|
