CDC15/YAR019C Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 53 5.4%
Arg[R] 33 3.4%
Asn[N] 64 6.6%
Asp[D] 58 6.0%
Cys[C] 13 1.3%
Gln[Q] 27 2.8%
Glu[E] 42 4.3%
Gly[G] 28 2.9%
His[H] 23 2.4%
Ile[I] 82 8.4%
Leu[L] 111 11.4%
Lys[K] 70 7.2%
Met[M] 17 1.7%
Phe[F] 51 5.2%
Pro[P] 42 4.3%
Ser[S] 103 10.6%
Thr[T] 60 6.2%
Trp[W] 11 1.1%
Tyr[Y] 33 3.4%
Val[V] 53 5.4%
Total Length 974

Instability index
Instability index (II) 45.53
This classifies the protein as unstable

Atomic composition
Carbon 4987
Hydrogen 7845
Nitrogen 1291
Oxygen 1462
Sulfur 30
Total number of atoms 15615
Formula = C4987H7845N1291O1462S30

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 110545 M-1 cm-1
assuming NO Cys residues appear as half cystines 109670 M-1 cm-1
assuming all Cys residues are reduced 109670 M-1 cm-1
assuming all pairs of Cys residues form cystines 110420 M-1 cm-1

Aliphatic index
Aliphatic index 98.50

Coding Region Translation Calculations
Codon Bias 0.000
Codon Adaptation Index 0.000
Frequency of Optimal Codons 0.000
Hydropathicity of Protein 0.000
Aromaticity Score 0.000