Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
53 |
5.4% |
| Arg[R] |
33 |
3.4% |
| Asn[N] |
64 |
6.6% |
| Asp[D] |
58 |
6.0% |
| Cys[C] |
13 |
1.3% |
| Gln[Q] |
27 |
2.8% |
| Glu[E] |
42 |
4.3% |
| Gly[G] |
28 |
2.9% |
| His[H] |
23 |
2.4% |
| Ile[I] |
82 |
8.4% |
| Leu[L] |
111 |
11.4% |
| Lys[K] |
70 |
7.2% |
| Met[M] |
17 |
1.7% |
| Phe[F] |
51 |
5.2% |
| Pro[P] |
42 |
4.3% |
| Ser[S] |
103 |
10.6% |
| Thr[T] |
60 |
6.2% |
| Trp[W] |
11 |
1.1% |
| Tyr[Y] |
33 |
3.4% |
| Val[V] |
53 |
5.4% |
| Total Length |
974 |
|
| Instability index (II) |
45.53 |
| This classifies the protein as unstable |
|
| Carbon |
4987 |
| Hydrogen |
7845 |
| Nitrogen |
1291 |
| Oxygen |
1462 |
| Sulfur |
30 |
| Total number of atoms |
15615 |
| Formula = C4987H7845N1291O1462S30 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
110545 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
109670 M-1 cm-1 |
| assuming all Cys residues are reduced |
109670 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
110420 M-1 cm-1 |
|
|
| Codon Bias |
0.000 |
| Codon Adaptation Index |
0.000 |
| Frequency of Optimal Codons |
0.000 |
| Hydropathicity of Protein |
0.000 |
| Aromaticity Score |
0.000 |
|