YAR009C Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 47 3.9%
Arg[R] 53 4.4%
Asn[N] 77 6.4%
Asp[D] 70 5.9%
Cys[C] 11 0.9%
Gln[Q] 44 3.7%
Glu[E] 69 5.8%
Gly[G] 48 4.0%
His[H] 39 3.3%
Ile[I] 99 8.3%
Leu[L] 106 8.9%
Lys[K] 99 8.3%
Met[M] 22 1.8%
Phe[F] 36 3.0%
Pro[P] 68 5.7%
Ser[S] 112 9.4%
Thr[T] 86 7.2%
Trp[W] 10 0.8%
Tyr[Y] 56 4.7%
Val[V] 44 3.7%
Total Length 1196

Instability index
Instability index (II) 49.39
This classifies the protein as unstable

Atomic composition
Carbon 6087
Hydrogen 9595
Nitrogen 1663
Oxygen 1850
Sulfur 33
Total number of atoms 19228
Formula = C6087H9595N1663O1850S33

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 139065 M-1 cm-1
assuming NO Cys residues appear as half cystines 138440 M-1 cm-1
assuming all Cys residues are reduced 138440 M-1 cm-1
assuming all pairs of Cys residues form cystines 139065 M-1 cm-1

Aliphatic index
Aliphatic index 81.45

Coding Region Translation Calculations
Codon Bias 0.000
Codon Adaptation Index 0.000
Frequency of Optimal Codons 0.000
Hydropathicity of Protein 0.000
Aromaticity Score 0.000