SEO1/YAL067C Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 34 5.7%
Arg[R] 23 3.9%
Asn[N] 32 5.4%
Asp[D] 30 5.1%
Cys[C] 8 1.3%
Gln[Q] 18 3.0%
Glu[E] 29 4.9%
Gly[G] 30 5.1%
His[H] 9 1.5%
Ile[I] 46 7.8%
Leu[L] 57 9.6%
Lys[K] 39 6.6%
Met[M] 11 1.9%
Phe[F] 35 5.9%
Pro[P] 16 2.7%
Ser[S] 48 8.1%
Thr[T] 31 5.2%
Trp[W] 22 3.7%
Tyr[Y] 35 5.9%
Val[V] 40 6.7%
Total Length 593

Instability index
Instability index (II) 35.75
This classifies the protein as stable

Atomic composition
Carbon 3188
Hydrogen 4809
Nitrogen 791
Oxygen 876
Sulfur 19
Total number of atoms 9683
Formula = C3188H4809N791O876S19

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 173650 M-1 cm-1
assuming NO Cys residues appear as half cystines 173150 M-1 cm-1
assuming all Cys residues are reduced 173150 M-1 cm-1
assuming all pairs of Cys residues form cystines 173650 M-1 cm-1

Aliphatic index
Aliphatic index 93.04

Coding Region Translation Calculations
Codon Bias 0.135
Codon Adaptation Index 0.184
Frequency of Optimal Codons 0.495
Hydropathicity of Protein -0.063
Aromaticity Score 0.155