ACS1/YAL054C Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 56 7.9%
Arg[R] 30 4.2%
Asn[N] 23 3.2%
Asp[D] 48 6.7%
Cys[C] 7 1.0%
Gln[Q] 22 3.1%
Glu[E] 38 5.3%
Gly[G] 50 7.0%
His[H] 20 2.8%
Ile[I] 44 6.2%
Leu[L] 58 8.1%
Lys[K] 47 6.6%
Met[M] 8 1.1%
Phe[F] 26 3.6%
Pro[P] 40 5.6%
Ser[S] 51 7.2%
Thr[T] 47 6.6%
Trp[W] 12 1.7%
Tyr[Y] 31 4.3%
Val[V] 55 7.7%
Total Length 713

Instability index
Instability index (II) 34.64
This classifies the protein as stable

Atomic composition
Carbon 3568
Hydrogen 5537
Nitrogen 947
Oxygen 1060
Sulfur 15
Total number of atoms 11127
Formula = C3568H5537N947O1060S15

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 112565 M-1 cm-1
assuming NO Cys residues appear as half cystines 112190 M-1 cm-1
assuming all Cys residues are reduced 112190 M-1 cm-1
assuming all pairs of Cys residues form cystines 112565 M-1 cm-1

Aliphatic index
Aliphatic index 86.02

Coding Region Translation Calculations
Codon Bias 0.311
Codon Adaptation Index 0.255
Frequency of Optimal Codons 0.589
Hydropathicity of Protein -0.272
Aromaticity Score 0.097