GCV3/YAL044C Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 10 5.9%
Arg[R] 5 2.9%
Asn[N] 9 5.3%
Asp[D] 8 4.7%
Cys[C] 0 0.0%
Gln[Q] 8 4.7%
Glu[E] 17 10.0%
Gly[G] 14 8.2%
His[H] 3 1.8%
Ile[I] 6 3.5%
Leu[L] 16 9.4%
Lys[K] 9 5.3%
Met[M] 5 2.9%
Phe[F] 3 1.8%
Pro[P] 7 4.1%
Ser[S] 12 7.1%
Thr[T] 14 8.2%
Trp[W] 3 1.8%
Tyr[Y] 6 3.5%
Val[V] 15 8.8%
Total Length 170

Instability index
Instability index (II) 45.52
This classifies the protein as unstable

Atomic composition
Carbon 826
Hydrogen 1300
Nitrogen 220
Oxygen 270
Sulfur 5
Total number of atoms 2621
Formula = C826H1300N220O270S5

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 25440 M-1 cm-1

Aliphatic index
Aliphatic index 81.94

Coding Region Translation Calculations
Codon Bias 0.143
Codon Adaptation Index 0.164
Frequency of Optimal Codons 0.488
Hydropathicity of Protein -0.452
Aromaticity Score 0.071