Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
38 |
4.8% |
| Arg[R] |
29 |
3.7% |
| Asn[N] |
40 |
5.1% |
| Asp[D] |
48 |
6.1% |
| Cys[C] |
4 |
0.5% |
| Gln[Q] |
40 |
5.1% |
| Glu[E] |
51 |
6.5% |
| Gly[G] |
26 |
3.3% |
| His[H] |
12 |
1.5% |
| Ile[I] |
46 |
5.9% |
| Leu[L] |
104 |
13.2% |
| Lys[K] |
61 |
7.8% |
| Met[M] |
21 |
2.7% |
| Phe[F] |
29 |
3.7% |
| Pro[P] |
36 |
4.6% |
| Ser[S] |
92 |
11.7% |
| Thr[T] |
47 |
6.0% |
| Trp[W] |
7 |
0.9% |
| Tyr[Y] |
18 |
2.3% |
| Val[V] |
36 |
4.6% |
| Total Length |
785 |
|
| Instability index (II) |
50.59 |
| This classifies the protein as unstable |
|
| Carbon |
3926 |
| Hydrogen |
6308 |
| Nitrogen |
1044 |
| Oxygen |
1221 |
| Sulfur |
25 |
| Total number of atoms |
12524 |
| Formula = C3926H6308N1044O1221S25 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
65570 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
65320 M-1 cm-1 |
| assuming all Cys residues are reduced |
65320 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
65570 M-1 cm-1 |
|
|
| Codon Bias |
0.133 |
| Codon Adaptation Index |
0.130 |
| Frequency of Optimal Codons |
0.478 |
| Hydropathicity of Protein |
-0.363 |
| Aromaticity Score |
0.069 |
|
