Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
37 |
4.3% |
| Arg[R] |
34 |
4.0% |
| Asn[N] |
70 |
8.2% |
| Asp[D] |
36 |
4.2% |
| Cys[C] |
7 |
0.8% |
| Gln[Q] |
40 |
4.7% |
| Glu[E] |
54 |
6.3% |
| Gly[G] |
17 |
2.0% |
| His[H] |
25 |
2.9% |
| Ile[I] |
62 |
7.3% |
| Leu[L] |
84 |
9.8% |
| Lys[K] |
62 |
7.3% |
| Met[M] |
22 |
2.6% |
| Phe[F] |
41 |
4.8% |
| Pro[P] |
31 |
3.6% |
| Ser[S] |
127 |
14.9% |
| Thr[T] |
46 |
5.4% |
| Trp[W] |
6 |
0.7% |
| Tyr[Y] |
18 |
2.1% |
| Val[V] |
35 |
4.1% |
| Total Length |
854 |
|
| Instability index (II) |
50.72 |
| This classifies the protein as unstable |
|
| Carbon |
4264 |
| Hydrogen |
6782 |
| Nitrogen |
1184 |
| Oxygen |
1336 |
| Sulfur |
29 |
| Total number of atoms |
13595 |
| Formula = C4264H6782N1184O1336S29 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
60195 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
59820 M-1 cm-1 |
| assuming all Cys residues are reduced |
59820 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
60195 M-1 cm-1 |
|
|
| Codon Bias |
0.048 |
| Codon Adaptation Index |
0.128 |
| Frequency of Optimal Codons |
0.442 |
| Hydropathicity of Protein |
-0.478 |
| Aromaticity Score |
0.076 |
|
