Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
43 |
8.6% |
| Arg[R] |
24 |
4.8% |
| Asn[N] |
26 |
5.2% |
| Asp[D] |
32 |
6.4% |
| Cys[C] |
7 |
1.4% |
| Gln[Q] |
10 |
2.0% |
| Glu[E] |
28 |
5.6% |
| Gly[G] |
34 |
6.8% |
| His[H] |
7 |
1.4% |
| Ile[I] |
37 |
7.4% |
| Leu[L] |
35 |
7.0% |
| Lys[K] |
37 |
7.4% |
| Met[M] |
11 |
2.2% |
| Phe[F] |
15 |
3.0% |
| Pro[P] |
25 |
5.0% |
| Ser[S] |
27 |
5.4% |
| Thr[T] |
38 |
7.6% |
| Trp[W] |
1 |
0.2% |
| Tyr[Y] |
15 |
3.0% |
| Val[V] |
48 |
9.6% |
| Total Length |
500 |
|
| Instability index (II) |
23.23 |
| This classifies the protein as stable |
|
| Carbon |
2414 |
| Hydrogen |
3906 |
| Nitrogen |
660 |
| Oxygen |
737 |
| Sulfur |
18 |
| Total number of atoms |
7735 |
| Formula = C2414H3906N660O737S18 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
28225 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
27850 M-1 cm-1 |
| assuming all Cys residues are reduced |
27850 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
28225 M-1 cm-1 |
|
|
| Codon Bias |
0.882 |
| Codon Adaptation Index |
0.893 |
| Frequency of Optimal Codons |
0.930 |
| Hydropathicity of Protein |
-0.148 |
| Aromaticity Score |
0.062 |
|
