Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
11 |
3.8% |
| Arg[R] |
15 |
5.2% |
| Asn[N] |
16 |
5.5% |
| Asp[D] |
25 |
8.7% |
| Cys[C] |
2 |
0.7% |
| Gln[Q] |
9 |
3.1% |
| Glu[E] |
30 |
10.4% |
| Gly[G] |
9 |
3.1% |
| His[H] |
1 |
0.3% |
| Ile[I] |
17 |
5.9% |
| Leu[L] |
43 |
14.9% |
| Lys[K] |
32 |
11.1% |
| Met[M] |
6 |
2.1% |
| Phe[F] |
6 |
2.1% |
| Pro[P] |
7 |
2.4% |
| Ser[S] |
18 |
6.2% |
| Thr[T] |
15 |
5.2% |
| Trp[W] |
0 |
0.0% |
| Tyr[Y] |
8 |
2.8% |
| Val[V] |
19 |
6.6% |
| Total Length |
289 |
|
| Instability index (II) |
34.57 |
| This classifies the protein as stable |
|
| Carbon |
1464 |
| Hydrogen |
2423 |
| Nitrogen |
393 |
| Oxygen |
466 |
| Sulfur |
8 |
| Total number of atoms |
4754 |
| Formula = C1464H2423N393O466S8 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
12045 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
11920 M-1 cm-1 |
| assuming all Cys residues are reduced |
11920 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
12045 M-1 cm-1 |
|
|
| Codon Bias |
-0.055 |
| Codon Adaptation Index |
0.109 |
| Frequency of Optimal Codons |
0.375 |
| Hydropathicity of Protein |
-0.529 |
| Aromaticity Score |
0.048 |
|
