Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
66 |
5.8% |
| Arg[R] |
50 |
4.4% |
| Asn[N] |
82 |
7.3% |
| Asp[D] |
104 |
9.2% |
| Cys[C] |
7 |
0.6% |
| Gln[Q] |
46 |
4.1% |
| Glu[E] |
85 |
7.5% |
| Gly[G] |
38 |
3.4% |
| His[H] |
22 |
1.9% |
| Ile[I] |
64 |
5.7% |
| Leu[L] |
103 |
9.1% |
| Lys[K] |
102 |
9.0% |
| Met[M] |
22 |
1.9% |
| Phe[F] |
39 |
3.4% |
| Pro[P] |
50 |
4.4% |
| Ser[S] |
95 |
8.4% |
| Thr[T] |
63 |
5.6% |
| Trp[W] |
6 |
0.5% |
| Tyr[Y] |
32 |
2.8% |
| Val[V] |
55 |
4.9% |
| Total Length |
1131 |
|
| Instability index (II) |
45.66 |
| This classifies the protein as unstable |
|
| Carbon |
5617 |
| Hydrogen |
8929 |
| Nitrogen |
1561 |
| Oxygen |
1828 |
| Sulfur |
29 |
| Total number of atoms |
17964 |
| Formula = C5617H8929N1561O1828S29 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
81055 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
80680 M-1 cm-1 |
| assuming all Cys residues are reduced |
80680 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
81055 M-1 cm-1 |
|
|
| Codon Bias |
0.081 |
| Codon Adaptation Index |
0.163 |
| Frequency of Optimal Codons |
0.461 |
| Hydropathicity of Protein |
-0.767 |
| Aromaticity Score |
0.068 |
|
