Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
41 |
10.4% |
| Arg[R] |
13 |
3.3% |
| Asn[N] |
22 |
5.6% |
| Asp[D] |
21 |
5.3% |
| Cys[C] |
0 |
0.0% |
| Gln[Q] |
15 |
3.8% |
| Glu[E] |
21 |
5.3% |
| Gly[G] |
28 |
7.1% |
| His[H] |
14 |
3.6% |
| Ile[I] |
24 |
6.1% |
| Leu[L] |
40 |
10.2% |
| Lys[K] |
22 |
5.6% |
| Met[M] |
3 |
0.8% |
| Phe[F] |
14 |
3.6% |
| Pro[P] |
17 |
4.3% |
| Ser[S] |
29 |
7.4% |
| Thr[T] |
28 |
7.1% |
| Trp[W] |
2 |
0.5% |
| Tyr[Y] |
10 |
2.5% |
| Val[V] |
30 |
7.6% |
| Total Length |
394 |
|
| Instability index (II) |
30.47 |
| This classifies the protein as stable |
|
| Carbon |
1896 |
| Hydrogen |
3010 |
| Nitrogen |
522 |
| Oxygen |
583 |
| Sulfur |
3 |
| Total number of atoms |
6014 |
| Formula = C1896H3010N522O583S3 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
25900 M-1 cm-1 |
|
|
| Codon Bias |
0.548 |
| Codon Adaptation Index |
0.420 |
| Frequency of Optimal Codons |
0.728 |
| Hydropathicity of Protein |
-0.144 |
| Aromaticity Score |
0.066 |
|
