MDM10/YAL010C Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 17 3.4%
Arg[R] 20 4.1%
Asn[N] 38 7.7%
Asp[D] 29 5.9%
Cys[C] 5 1.0%
Gln[Q] 18 3.7%
Glu[E] 21 4.3%
Gly[G] 17 3.4%
His[H] 11 2.2%
Ile[I] 24 4.9%
Leu[L] 59 12.0%
Lys[K] 29 5.9%
Met[M] 6 1.2%
Phe[F] 28 5.7%
Pro[P] 18 3.7%
Ser[S] 66 13.4%
Thr[T] 44 8.9%
Trp[W] 8 1.6%
Tyr[Y] 20 4.1%
Val[V] 15 3.0%
Total Length 493

Instability index
Instability index (II) 35.98
This classifies the protein as stable

Atomic composition
Carbon 2510
Hydrogen 3870
Nitrogen 668
Oxygen 780
Sulfur 11
Total number of atoms 7839
Formula = C2510H3870N668O780S11

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 74050 M-1 cm-1
assuming NO Cys residues appear as half cystines 73800 M-1 cm-1
assuming all Cys residues are reduced 73800 M-1 cm-1
assuming all pairs of Cys residues form cystines 74050 M-1 cm-1

Aliphatic index
Aliphatic index 77.93

Coding Region Translation Calculations
Codon Bias 0.000
Codon Adaptation Index 0.000
Frequency of Optimal Codons 0.000
Hydropathicity of Protein 0.000
Aromaticity Score 0.000