Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
60 |
9.3% |
| Arg[R] |
26 |
4.0% |
| Asn[N] |
32 |
5.0% |
| Asp[D] |
49 |
7.6% |
| Cys[C] |
3 |
0.5% |
| Gln[Q] |
23 |
3.6% |
| Glu[E] |
51 |
7.9% |
| Gly[G] |
50 |
7.8% |
| His[H] |
5 |
0.8% |
| Ile[I] |
40 |
6.2% |
| Leu[L] |
48 |
7.5% |
| Lys[K] |
53 |
8.3% |
| Met[M] |
8 |
1.2% |
| Phe[F] |
28 |
4.4% |
| Pro[P] |
28 |
4.4% |
| Ser[S] |
41 |
6.4% |
| Thr[T] |
45 |
7.0% |
| Trp[W] |
1 |
0.2% |
| Tyr[Y] |
10 |
1.6% |
| Val[V] |
41 |
6.4% |
| Total Length |
642 |
|
| Instability index (II) |
32.09 |
| This classifies the protein as stable |
|
| Carbon |
3056 |
| Hydrogen |
4905 |
| Nitrogen |
839 |
| Oxygen |
994 |
| Sulfur |
11 |
| Total number of atoms |
9805 |
| Formula = C3056H4905N839O994S11 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
20525 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
20400 M-1 cm-1 |
| assuming all Cys residues are reduced |
20400 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
20525 M-1 cm-1 |
|
|
| Codon Bias |
0.763 |
| Codon Adaptation Index |
0.712 |
| Frequency of Optimal Codons |
0.859 |
| Hydropathicity of Protein |
-0.438 |
| Aromaticity Score |
0.061 |
|
