Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
52 |
4.5% |
| Arg[R] |
64 |
5.5% |
| Asn[N] |
66 |
5.7% |
| Asp[D] |
79 |
6.8% |
| Cys[C] |
7 |
0.6% |
| Gln[Q] |
37 |
3.2% |
| Glu[E] |
73 |
6.3% |
| Gly[G] |
59 |
5.1% |
| His[H] |
16 |
1.4% |
| Ile[I] |
87 |
7.5% |
| Leu[L] |
97 |
8.4% |
| Lys[K] |
125 |
10.8% |
| Met[M] |
17 |
1.5% |
| Phe[F] |
42 |
3.6% |
| Pro[P] |
30 |
2.6% |
| Ser[S] |
97 |
8.4% |
| Thr[T] |
75 |
6.5% |
| Trp[W] |
15 |
1.3% |
| Tyr[Y] |
35 |
3.0% |
| Val[V] |
87 |
7.5% |
| Total Length |
1160 |
|
| Instability index (II) |
31.94 |
| This classifies the protein as stable |
|
| Carbon |
5878 |
| Hydrogen |
9475 |
| Nitrogen |
1627 |
| Oxygen |
1775 |
| Sulfur |
24 |
| Total number of atoms |
18779 |
| Formula = C5878H9475N1627O1775S24 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
135025 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
134650 M-1 cm-1 |
| assuming all Cys residues are reduced |
134650 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
135025 M-1 cm-1 |
|
|
| Codon Bias |
-0.019 |
| Codon Adaptation Index |
0.121 |
| Frequency of Optimal Codons |
0.409 |
| Hydropathicity of Protein |
-0.512 |
| Aromaticity Score |
0.079 |
|
