PEF1/YGR058W Protein Information

PEF1 BASIC PROTEIN INFORMATION

Standard Name	Pef1p 1
Systematic Name	Ygr058wp
ORF Classification	Verified
Description	Penta-EF-hand protein required for polar bud growth and cell wall abscission; binds calcium and zinc with different affinity; localizes to bud site in G1, bud neck in G2 ( 1 )
Name Description	Penta-EF-Hand protein 1

Experimental Data
Molecules/cell	1630 2

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	335
Molecular Weight (Da)	38,390
Isoelectric Point (pI)	10.12

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Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Pef1p (InterPro)
Physical Interactions	There are 14 total curated physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| PSI-BLAST \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
Secondary Structure Prediction	GCG:PepPlot \| GCG:PepStruct \| GCG:Helical Wheel
External Sequence Databases	EBI: P53238 \| UPI000013AF45 MIPS: YGR058W NCBI: NP_011572.1 \| 6321495

Amino Acid Sequence (or in FASTA format)
1 MCAKKLKYAA GDDFVRYATP KEAMEETRRE FEKEKQRQQQ IKVTQAQTPN 51 TRVHSAPIPL QTQYNKNRAE NGHHSYGSPQ SYSPRHTKTP VDPRYNVIAQ 101 KPAGRPIPPA PTHYNNLNTS AQRIASSPPP LIHNQAVPAQ LLKKVAPASF 151 DSREDVRDMQ VATQLFHNHD VKGKNRLTAE ELQNLLQNDD NSHFCISSVD 201 ALINLFGASR FGTVNQAEFI ALYKRVKSWR KVYVDNDING SLTISVSEFH 251 NSLQELGYLI PFEVSEKTFD QYAEFINRNG TGKELKFDKF VEALVWLMRL 301 TKLFRKFDTN QEGIATIQYK DFIYATLYLG RFLPH*

Amino Acid Sequence (or in FASTA format)

       1  MCAKKLKYAA GDDFVRYATP KEAMEETRRE FEKEKQRQQQ IKVTQAQTPN
      51  TRVHSAPIPL QTQYNKNRAE NGHHSYGSPQ SYSPRHTKTP VDPRYNVIAQ
     101  KPAGRPIPPA PTHYNNLNTS AQRIASSPPP LIHNQAVPAQ LLKKVAPASF
     151  DSREDVRDMQ VATQLFHNHD VKGKNRLTAE ELQNLLQNDD NSHFCISSVD
     201  ALINLFGASR FGTVNQAEFI ALYKRVKSWR KVYVDNDING SLTISVSEFH
     251  NSLQELGYLI PFEVSEKTFD QYAEFINRNG TGKELKFDKF VEALVWLMRL
     301  TKLFRKFDTN QEGIATIQYK DFIYATLYLG RFLPH*

EXTERNAL LINKS for Pef1p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YGAC Triples
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)
Candida (CGD)	BioPIXIE	MIPS
Candida (CandidaDB)	CYC2008 (complexes)	Pfam domains
YGOB	DIP	PhosphoPep Database
YOGY		YeastRC Structure Prediction (Seattle)

REFERENCES CITED ON THIS PAGE [View Complete Literature Guide for Pef1p ]

1)	Vernarecci S, et al. (2007) The yeast penta-EF protein Pef1p is involved in cation-dependent budding and cell polarization. Mol Microbiol 65(4):1122-38
2)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41

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