SAE2/YGL175C Protein Information Help

SAE2 BASIC PROTEIN INFORMATION

Standard Name Sae2p 1
Systematic Name Ygl175cp
Alias Com1p 2
ORF Classification Verified
Description Endonuclease that processes hairpin DNA structures with the MRX complex; involved in meiotic and mitotic double-strand break repair; phosphorylated in response to DNA damage and required for normal resistance to DNA-damaging agents ( 1, 2, 3, 4, 5 )
Name Description Sporulation in the Absence of spo Eleven 1
Experimental Data
Molecules/cell 1030 6
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 345
Molecular Weight (Da) 40,097
Isoelectric Point (pI) 6.54

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Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Sae2p (InterPro)
Physical Interactions There are 12 total curated physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | PSI-BLAST | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
Secondary Structure Prediction GCG:PepPlot | GCG:PepStruct | GCG:Helical Wheel
External Sequence Databases EBI: P46946 | UPI00001354DE
MIPS: YGL175C
NCBI: NP_011340.1 | 6321263
Amino Acid Sequence (or in FASTA format)
 
       1  MVTGEENVYL KSSLSILKEL SLDELLNVQY DVTTLIAKRV QALQNRNKCV
      51  LEEPNSKLAE ILCHEKNAPQ QSSQTSAGPG EQDSEDFILT QFDEDIKKES
     101  AEVHYRNENK HTVQLPLVTM PPNRHKRKIS EFSSPLNGLN NLSDLEDCSD
     151  TVIHEKDNDK ENKTRKLLGI ELENPESTSP NLYKNVKDNF LFDFNTNPLT
     201  KRAWILEDFR PNEDIAPVKR GRRKLERFYA QVGKPEDSKH RSLSVVIESQ
     251  NSDYEFAFDN LRNRSKSPPG FGRLDFPSTQ EGNEDKKKSQ EIIRRKTKYR
     301  FLMASNNKIP PYEREYVFKR EQLNQIVDDG CFFWSDKLLQ IYARC*

EXTERNAL LINKS for Sae2p

Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily YGAC Triples
Ashbya (AGD) BOND GPMdb (Mass Spec.)
YGOB BioPIXIE MIPS
YOGY CYC2008 (complexes) Pfam domains

DIP PhosphoPep Database

REFERENCES CITED ON THIS PAGE [View Complete Literature Guide for Sae2p ]

1) McKee AH and Kleckner N  (1997) A general method for identifying recessive diploid-specific mutations in Saccharomyces cerevisiae, its application to the isolation of mutants blocked at intermediate stages of meiotic prophase and characterization of a new gene SAE2. Genetics 146(3):797-816
2) Prinz S, et al.  (1997) Isolation of COM1, a new gene required to complete meiotic double-strand break-induced recombination in Saccharomyces cerevisiae. Genetics 146(3):781-95
3) Rattray AJ, et al.  (2001) Fidelity of mitotic double-strand-break repair in Saccharomyces cerevisiae: a role for SAE2/COM1. Genetics 158(1):109-22
4) Baroni E, et al.  (2004) The functions of budding yeast Sae2 in the DNA damage response require Mec1- and Tel1-dependent phosphorylation. Mol Cell Biol 24(10):4151-65
5) Lengsfeld BM, et al.  (2007) Sae2 Is an Endonuclease that Processes Hairpin DNA Cooperatively with the Mre11/Rad50/Xrs2 Complex. Mol Cell 28(4):638-51
6) Ghaemmaghami S, et al.  (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41