DOT1/YDR440W Protein Information Help

DOT1 BASIC PROTEIN INFORMATION

Standard Name Dot1p 1
Systematic Name Ydr440wp
Alias Pch1p 2
ORF Classification Verified
Description Nucleosomal histone H3-Lys79 methylase; methylation is required for telomeric silencing, meiotic checkpoint control, and DNA damage response ( 2, 3, 4, 5 )
Name Description Disruptor Of Telomeric silencing 1, 6
Gene Product Kmt4 7
Experimental Data
Molecules/cell 2160 8
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 582
Molecular Weight (Da) 66,201
Isoelectric Point (pI) 9.76

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Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Dot1p (InterPro)
Physical Interactions There are 28 total curated physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | PSI-BLAST | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
Secondary Structure Prediction GCG:PepPlot | GCG:PepStruct | GCG:Helical Wheel
External Sequence Databases EBI: Q04089 | UPI00001296B4
MIPS: YDR440W
NCBI: NP_010728.1 | 6320648
External Classifications EC: 2.1.1.43 [Histone-lysine N-methyltransferase]
Amino Acid Sequence (or in FASTA format)
 
       1  MGGQESISNN NSDSFIMSSP NLDSQESSIS PIDEKKGTDM QTKSLSSYSK
      51  GTLLSKQVQN LLEEANKYDP IYGSSLPRGF LRDRNTKGKD NGLVPLVEKV
     101  IPPIHKKTNN RNTRKKSSTT TKKDVKKPKA AKVKGKNGRT NHKHTPISKQ
     151  EIDTAREKKP LKKGRANKKN DRDSPSSTFV DWNGPCLRLQ YPLFDIEYLR
     201  SHEIYSGTPI QSISLRTNSP QPTSLTSDND TSSVTTAKLQ SILFSNYMEE
     251  YKVDFKRSTA IYNPMSEIGK LIEYSCLVFL PSPYAEQLKE TILPDLNASF
     301  DNSDTKGFVN AINLYNKMIR EIPRQRIIDH LETIDKIPRS FIHDFLHIVY
     351  TRSIHPQANK LKHYKAFSNY VYGELLPNFL SDVYQQCQLK KGDTFMDLGS
     401  GVGNCVVQAA LECGCALSFG CEIMDDASDL TILQYEELKK RCKLYGMRLN
     451  NVEFSLKKSF VDNNRVAELI PQCDVILVNN FLFDEDLNKK VEKILQTAKV
     501  GCKIISLKSL RSLTYQINFY NVENIFNRLK VQRYDLKEDS VSWTHSGGEY
     551  YISTVMEDVD ESLFSPAARG RRNRGTPVKY TR*

EXTERNAL LINKS for Dot1p

Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily YGAC Triples
Ashbya (AGD) BOND GPMdb (Mass Spec.)
YGOB BioPIXIE MIPS
YOGY CYC2008 (complexes) Pfam domains

DIP PhosphoPep Database

YeastRC Two-Hybrid (Seattle) YeastRC Structure Prediction (Seattle)

REFERENCES CITED ON THIS PAGE [View Complete Literature Guide for Dot1p ]

1) Singer MS, et al.  (1998) Identification of high-copy disruptors of telomeric silencing in Saccharomyces cerevisiae. Genetics 150(2):613-32
2) San-Segundo PA and Roeder GS  (2000) Role for the silencing protein Dot1 in meiotic checkpoint control. Mol Biol Cell 11(10):3601-15
3) van Leeuwen F, et al.  (2002) Dot1p modulates silencing in yeast by methylation of the nucleosome core. Cell 109(6):745-56
4) Game JC, et al.  (2005) X-ray survival characteristics and genetic analysis for nine Saccharomyces deletion mutants that show altered radiation sensitivity. Genetics 169(1):51-63
5) Wysocki R, et al.  (2005) Role of Dot1-dependent histone H3 methylation in G1 and S phase DNA damage checkpoint functions of Rad9. Mol Cell Biol 25(19):8430-43
6) Lacoste N, et al.  (2002) Disruptor of telomeric silencing-1 is a chromatin-specific histone H3 methyltransferase. J Biol Chem 277(34):30421-4
7) Allis CD, et al.  (2007) New nomenclature for chromatin-modifying enzymes. Cell 131(4):633-6
8) Ghaemmaghami S, et al.  (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41