DAD3/YBR233W-A Protein Information Help

Standard Name Dad3p 1
Systematic Name Ybr233w-ap
ORF Classification Verified
Description Essential subunit of the Dam1 complex (aka DASH complex); complex couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis (2, 3, 4, 5, 6)
Name Description Duo1 And Dam1 interacting 1
Experimental Data
Molecules/cell 468 7
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 94
Molecular Weight (Da) 10,848
Isoelectric Point (pI) 5.25

Click on image for expanded interactive view
pbrowse

Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Dad3p (InterPro)
Physical Interactions There are 22 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI00001682C5 | P69850
MIPS: YBR233W-A
NCBI: 21450619 | 67460131 | NP_631876.1 | NM_001184461.1
GenBank/EMBL/DDBJ: DAA07349.1 | Z36103
Amino Acid Sequence (or in FASTA format)
       1  MEHNLSPLQQ EVLDKYKQLS LDLKALDETI KELNYSQHRQ QHSQQETVSP
      51  DEILQEMRDI EVKIGLVGTL LKGSVYSLIL QRKQEQESLG SNSK*     

external links for Dad3p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily LoQate
Ashbya (AGD) BOND GPMdb (Mass Spec.) YPL+
Candida (CGD) CYC2008 (complexes) MIPS YeastGFP
YGOB Complexome Pfam domains YeastRC Public Image Repository
YOGY GeneMANIA


IMP


YeastRC Mass Spec (Seattle)

References cited on this page View Complete Literature Guide for Dad3p
1) Cheeseman IM, et al.  (2002) Phospho-regulation of kinetochore-microtubule attachments by the Aurora kinase Ipl1p. Cell 111(2):163-72
2) Hofmann C, et al.  (1998) Saccharomyces cerevisiae Duo1p and Dam1p, novel proteins involved in mitotic spindle function. J Cell Biol 143(4):1029-40
3) Li Y, et al.  (2002) The mitotic spindle is required for loading of the DASH complex onto the kinetochore. Genes Dev 16(2):183-97
4) Miranda JJ, et al.  (2005) The yeast DASH complex forms closed rings on microtubules. Nat Struct Mol Biol 12(2):138-43
5) Westermann S, et al.  (2005) Formation of a dynamic kinetochore- microtubule interface through assembly of the Dam1 ring complex. Mol Cell 17(2):277-90
6) Westermann S, et al.  (2006) The Dam1 kinetochore ring complex moves processively on depolymerizing microtubule ends. Nature 440(7083):565-9
7) Ghaemmaghami S, et al.  (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41