BI3/Q0115 Protein Information

Standard Name	Bi3p
Systematic Name	Q0115p
ORF Classification	Verified
Description	Mitochondrial mRNA maturase, forms a complex with Mrs1p to mediate splicing of the bI3 intron of the COB gene; encoded by both exon and intron sequences of partially processed COB mRNA (1, 2)

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	517
Molecular Weight (Da)	61,172
Isoelectric Point (pI)	10.4

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Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Bi3p (InterPro)
Transmembrane Domains	There are 6 total predicted transmembrane domains (TMHMM)
Signal Peptide(s)	There are 1 total predicted predicted signal peptide(s) (SignalP)
Physical Interactions	There are 4 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI000012ED31 \| Q9ZZW7 MIPS: Q0115 NCBI: 1334608 \| 4160378 \| 6225661 \| 6226531 \| 75766112 \| 75766113 \| NP_009317.1 GenBank/EMBL/DDBJ: AJ011856 \| X14637

Amino Acid Sequence (or in FASTA format)
1 MAFRKSNVYL SLVNSYIIDS PQPSSINYWW NMGSLLGLCL VIQIVTGIFM 51 AMHYSSNIEL AFSSVEHIMR DVHNGYILRY LHANGASFFF MVMFMHMAKG 101 LYYGSYRSPR VTLWNVGVII FILTIATAFL GYCCVYGQMS HWGATVITNL 151 FSAIPFVGND IVSWLWGGFN MEDPYYSNMM LNKSVLCWNI FIWMMNYYII 201 QLIIYNNMIW NKNNMVKMFI MRRKLAVINM YMYMKLIIQR TYSYYMNNTI 251 IYDKNHKLNT DNPIYAYIGG LFEGDGWITI SKKGKYLLYE LGIEMHIRDI 301 QLLYKIKNIL GIGKVTIKKL KMKDGTIKEM CKFNVRNKNH LKNIIIPIFD 351 KYPMLTNKHY DYLYFKDNLL KDIKYYNDLS YYLRPIKPFN TTEDILNKNY 401 FSSWLIGFFE AESCFSIYKP MNKKMKTASF EVSQNNSMEV MLAIKSYLKI 451 TQNIYTDKFN NSRMTTKSIN GIKNVVMFIN NNPIKLLGYK KLQYLLFLKD 501 LRTITKYNNY FKIPPKY*

Amino Acid Sequence (or in FASTA format)

       1  MAFRKSNVYL SLVNSYIIDS PQPSSINYWW NMGSLLGLCL VIQIVTGIFM
      51  AMHYSSNIEL AFSSVEHIMR DVHNGYILRY LHANGASFFF MVMFMHMAKG
     101  LYYGSYRSPR VTLWNVGVII FILTIATAFL GYCCVYGQMS HWGATVITNL
     151  FSAIPFVGND IVSWLWGGFN MEDPYYSNMM LNKSVLCWNI FIWMMNYYII
     201  QLIIYNNMIW NKNNMVKMFI MRRKLAVINM YMYMKLIIQR TYSYYMNNTI
     251  IYDKNHKLNT DNPIYAYIGG LFEGDGWITI SKKGKYLLYE LGIEMHIRDI
     301  QLLYKIKNIL GIGKVTIKKL KMKDGTIKEM CKFNVRNKNH LKNIIIPIFD
     351  KYPMLTNKHY DYLYFKDNLL KDIKYYNDLS YYLRPIKPFN TTEDILNKNY
     401  FSSWLIGFFE AESCFSIYKP MNKKMKTASF EVSQNNSMEV MLAIKSYLKI
     451  TQNIYTDKFN NSRMTTKSIN GIKNVVMFIN NNPIKLLGYK KLQYLLFLKD
     501  LRTITKYNNY FKIPPKY*

external links for Bi3p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
Aspergillus (AspGD)	BioPIXIE	MIPS
YGOB	CYC2008 (complexes)	Pfam domains
YOGY	Complexome
	GeneMANIA

References cited on this page View Complete Literature Guide for Bi3p

1)	Lazowska J, et al. (1989) Protein encoded by the third intron of cytochrome b gene in Saccharomyces cerevisiae is an mRNA maturase. Analysis of mitochondrial mutants, RNA transcripts proteins and evolutionary relationships. J Mol Biol 205(2):275-89
2)	Bassi GS and Weeks KM (2003) Kinetic and thermodynamic framework for assembly of the six-component bI3 group I intron ribonucleoprotein catalyst. Biochemistry 42(33):9980-8