AI4/Q0065 Protein Information

Standard Name	Ai4p
Systematic Name	Q0065p
ORF Classification	Verified
Description	Endonuclease I-SceII, encoded by a mobile group I intron within the mitochondrial COX1 gene; intron is normally spliced by the BI4p maturase but AI4p can mutate to acquire the same maturase activity (1, 2)
Gene Product	I-SceII

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	556
Molecular Weight (Da)	63,311
Isoelectric Point (pI)	10.47

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Ai4p (InterPro)
Transmembrane Domains	There are 6 total predicted transmembrane domains (TMHMM)
Signal Peptide(s)	There are 1 total predicted predicted signal peptide(s) (SignalP)
Physical Interactions	There are 1 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI0000174BFB \| P03878 MIPS: Q0065 NCBI: 1334438 \| 13524 \| 243425 \| 3980164 \| 4160370 \| 50403710 \| 85666123 \| NP_009307.2 GenBank/EMBL/DDBJ: AJ011856 \| S76641 \| V00694 \| V00703
External Classifications	EC: 3.1.-.- [Hydrolases acting on ester bonds ]

Amino Acid Sequence (or in FASTA format)
1 MVQRWLYSTN AKDIAVLYFM LAIFSGMAGT AMSLIIRLEL AAPGSQYLHG 51 NSQLFNVLVV GHAVLMIFFL VMPALIGGFG NYLLPLMIGA TDTAFPRINN 101 IAFWVLPMGL VCLVTSTLVE SGAGTGWTVY PPLSSIQAHS GPSVDLAIFA 151 LHLTSISSLL GAINFIVTTL NMRTNGMTMH KLPLFVWSIF ITAFLLLLSL 201 PVLSAGITML LLDRNFNTSF FEVSGGGDPI LYEHLFWFFG QTVATIIMLM 251 MYNDMHFSKC WKLLKKWITN IMSTLFKALF VKMFMSYNNQ QDKMMNNTML 301 KKDNIKRSSE TTRKMLNNSM NKKFNQWLAG LIDGDGYFGI VSKKYVSLEI 351 TVALEDEMAL KEIQNKFGGS IKLRSGVKAI RYRLTNKTGM IKLINAVNGN 401 IRNTKRLVQF NKVCILLGID FIYPIKLTKD NSWFVGFFDA DGTINYSFKN 451 NHPQLTISVT NKYLQDVQEY KNILGGNIYF DKSQNGYYKW SIQSKDMVLN 501 FINDYIKMNP SRTTKMNKLY LSKEFYNLKE LKAYNKSSDS MQYKAWLNFE 551 NKWKNK*

Amino Acid Sequence (or in FASTA format)

       1  MVQRWLYSTN AKDIAVLYFM LAIFSGMAGT AMSLIIRLEL AAPGSQYLHG
      51  NSQLFNVLVV GHAVLMIFFL VMPALIGGFG NYLLPLMIGA TDTAFPRINN
     101  IAFWVLPMGL VCLVTSTLVE SGAGTGWTVY PPLSSIQAHS GPSVDLAIFA
     151  LHLTSISSLL GAINFIVTTL NMRTNGMTMH KLPLFVWSIF ITAFLLLLSL
     201  PVLSAGITML LLDRNFNTSF FEVSGGGDPI LYEHLFWFFG QTVATIIMLM
     251  MYNDMHFSKC WKLLKKWITN IMSTLFKALF VKMFMSYNNQ QDKMMNNTML
     301  KKDNIKRSSE TTRKMLNNSM NKKFNQWLAG LIDGDGYFGI VSKKYVSLEI
     351  TVALEDEMAL KEIQNKFGGS IKLRSGVKAI RYRLTNKTGM IKLINAVNGN
     401  IRNTKRLVQF NKVCILLGID FIYPIKLTKD NSWFVGFFDA DGTINYSFKN
     451  NHPQLTISVT NKYLQDVQEY KNILGGNIYF DKSQNGYYKW SIQSKDMVLN
     501  FINDYIKMNP SRTTKMNKLY LSKEFYNLKE LKAYNKSSDS MQYKAWLNFE
     551  NKWKNK*

external links for Ai4p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
Candida (CGD)	BioPIXIE	MIPS
YGOB	CYC2008 (complexes)	Pfam domains
YOGY	Complexome
	GeneMANIA

References cited on this page View Complete Literature Guide for Ai4p

1)	Wernette CM, et al. (1990) Purification of a site-specific endonuclease, I-Sce II, encoded by intron 4 alpha of the mitochondrial coxI gene of Saccharomyces cerevisiae. J Biol Chem 265(31):18976-82
2)	Dujardin G, et al. (1982) Single base substitution in an intron of oxidase gene compensates splicing defects of the cytochrome b gene. Nature 298(5875):628-32