DID2/YKR035W-A Protein Information

Standard Name	Did2p
Systematic Name	Ykr035w-ap
Alias	Fti1p , Chm1p , Vps46p 1 , Vpl30p 1
ORF Classification	Verified
Description	Class E protein of the vacuolar protein-sorting (Vps) pathway; binds Vps4p and directs it to dissociate ESCRT-III complexes; forms a functional and physical complex with Ist1p; human ortholog may be altered in breast tumors (2, 3, 4)
Name Description	Doa4-Independent Degradation 2

Experimental Data
Molecules/cell	2440 5

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	204
Molecular Weight (Da)	23,091
Isoelectric Point (pI)	6.87

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Did2p (InterPro)
Physical Interactions	There are 53 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI0000168389 \| P69771 MIPS: YKR035W-A NCBI: 257471798 \| 257471799 \| 257471800 \| 257471801 \| 398365065 \| 62899918 \| NP_012961.3 GenBank/EMBL/DDBJ: DAA09188.1 \| Z28260

Amino Acid Sequence (or in FASTA format)
1 MSRNSAAGLE NTLFQLKFTS KQLQKQANKA SKEEKQETNK LKRALNENED 51 ISRIYASNAI RKKNERLQLL KLASRVDSVA SRVQTAVTMR QVSASMGQVC 101 KGMDKALQNM NLQQITMIMD KFEQQFEDLD TSVNVYEDMG VNSDAMLVDN 151 DKVDELMSKV ADENGMELKQ SAKLDNVPEI KAKEVNVDDE KEDKLAQRLR 201 ALRG*

Amino Acid Sequence (or in FASTA format)

       1  MSRNSAAGLE NTLFQLKFTS KQLQKQANKA SKEEKQETNK LKRALNENED
      51  ISRIYASNAI RKKNERLQLL KLASRVDSVA SRVQTAVTMR QVSASMGQVC
     101  KGMDKALQNM NLQQITMIMD KFEQQFEDLD TSVNVYEDMG VNSDAMLVDN
     151  DKVDELMSKV ADENGMELKQ SAKLDNVPEI KAKEVNVDDE KEDKLAQRLR
     201  ALRG*

external links for Did2p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
Candida (CGD)	BioPIXIE	MIPS	YeastRC Public Image Repository
Candida (CandidaDB)	CYC2008 (complexes)	Pfam domains
YGOB	Complexome	YeastRC Structure Prediction (Seattle)
YOGY	GeneMANIA

References cited on this page View Complete Literature Guide for Did2p

1)	Raymond CK, et al. (1992) Morphological classification of the yeast vacuolar protein sorting mutants: evidence for a prevacuolar compartment in class E vps mutants. Mol Biol Cell 3(12):1389-402
2)	Amerik AY, et al. (2000) The Doa4 deubiquitinating enzyme is functionally linked to the vacuolar protein-sorting and endocytic pathways. Mol Biol Cell 11(10):3365-80
3)	Nickerson DP, et al. (2006) Did2 coordinates Vps4-mediated dissociation of ESCRT-III from endosomes. J Cell Biol 175(5):715-20
4)	Rue SM, et al. (2008) Novel ist1-did2 complex functions at a late step in multivesicular body sorting. Mol Biol Cell 19(2):475-84
5)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41