PZF1/YPR186C Protein Information

Standard Name	Pzf1p 1
Systematic Name	Ypr186cp
Alias	Tfc2p 2
ORF Classification	Verified
Description	Transcription factor IIIA (TFIIIA); essential DNA binding protein required for transcription of 5S rRNA by RNA polymerase III; not involved in transcription of other RNAP III genes; nine conserved zinc fingers; may also bind 5S rRNA (1, 2, 3, 4)
Name Description	Putative Zinc Finger 1

Experimental Data
Molecules/cell	1380 5

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	429
Molecular Weight (Da)	50,027
Isoelectric Point (pI)	8.52

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Pzf1p (InterPro)
Physical Interactions	There are 2 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI0000136C54 \| P39933 MIPS: YPR186C NCBI: 172903 \| 6325444 \| 730931 \| 786305 \| NP_015512.1 \| NM_001184283.1 GenBank/EMBL/DDBJ: DAA11602.1 \| M80611 \| M90638 \| U25841

Amino Acid Sequence (or in FASTA format)
1 MGGEVLNNEG MPLAELKQET IPISRSESSE SLNSLTSTRS SSSNRPKTYF 51 CDYDGCDKAF TRPSILTEHQ LSVHQGLRAF QCDKCAKSFV KKSHLERHLY 101 THSDTKPFQC SYCGKGVTTR QQLKRHEVTH TKSFICPEEG CNLRFYKHPQ 151 LRAHILSVHL HKLTCPHCNK SFQRPYRLRN HISKHHDPEV ENPYQCTFAG 201 CCKEFRIWSQ LQSHIKNDHP KLKCPICSKP CVGENGLQMH MIIHDDSLVT 251 KNWKCHICPD MSFSRKHDLL THYGSIHTEE DIPLELKYKI SDIQQLVQDH 301 GVQLGNSKHS NEQDEEKISN RLRKRRKLTE NNNVEFLQNE VDLEKRLESG 351 ENGLNLLLNT VGRKYRCFYN NCSRTFKTKE KYEKHIDKHK VHELKLKILQ 401 EKEENKTLVD QNHKEPFIIQ KETQSAGDK*

Amino Acid Sequence (or in FASTA format)

       1  MGGEVLNNEG MPLAELKQET IPISRSESSE SLNSLTSTRS SSSNRPKTYF
      51  CDYDGCDKAF TRPSILTEHQ LSVHQGLRAF QCDKCAKSFV KKSHLERHLY
     101  THSDTKPFQC SYCGKGVTTR QQLKRHEVTH TKSFICPEEG CNLRFYKHPQ
     151  LRAHILSVHL HKLTCPHCNK SFQRPYRLRN HISKHHDPEV ENPYQCTFAG
     201  CCKEFRIWSQ LQSHIKNDHP KLKCPICSKP CVGENGLQMH MIIHDDSLVT
     251  KNWKCHICPD MSFSRKHDLL THYGSIHTEE DIPLELKYKI SDIQQLVQDH
     301  GVQLGNSKHS NEQDEEKISN RLRKRRKLTE NNNVEFLQNE VDLEKRLESG
     351  ENGLNLLLNT VGRKYRCFYN NCSRTFKTKE KYEKHIDKHK VHELKLKILQ
     401  EKEENKTLVD QNHKEPFIIQ KETQSAGDK*

external links for Pzf1p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	Organelle DB
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YPL+
Candida (CGD)	BioPIXIE	MIPS	YeastGFP
Candida (CandidaDB)	CYC2008 (complexes)	Pfam domains	YeastRC Public Image Repository
YGOB	Complexome	YeastRC Structure Prediction (Seattle)
YOGY	GeneMANIA

References cited on this page View Complete Literature Guide for Pzf1p

1)	Woychik NA and Young RA (1992) Genes encoding transcription factor IIIA and the RNA polymerase common subunit RPB6 are divergently transcribed in Saccharomyces cerevisiae. Proc Natl Acad Sci U S A 89(9):3999-4003
2)	Archambault J, et al. (1992) The deduced sequence of the transcription factor TFIIIA from Saccharomyces cerevisiae reveals extensive divergence from Xenopus TFIIIA. J Biol Chem 267(5):3282-8
3)	Shastry BS (1996) Transcription factor IIIA (TFIIIA) in the second decade. J Cell Sci 109 ( Pt 3):535-9
4)	Wang CK and Weil PA (1989) Purification and characterization of Saccharomyces cerevisiae transcription factor IIIA. J Biol Chem 264(2):1092-9
5)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41