DPM1/YPR183W Protein Information

Standard Name	Dpm1p 1
Systematic Name	Ypr183wp
Alias	Sed3p
ORF Classification	Verified
Description	Dolichol phosphate mannose (Dol-P-Man) synthase of the ER membrane, catalyzes the formation of Dol-P-Man from Dol-P and GDP-Man; required for glycosyl phosphatidylinositol membrane anchoring, O mannosylation, and protein glycosylation (1, 2, 3, 4)
Name Description	Dolichol Phosphate Mannose synthase 1

Experimental Data
Molecules/cell	1890 5

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	267
Molecular Weight (Da)	30,362
Isoelectric Point (pI)	7.97

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Dpm1p (InterPro)
Transmembrane Domains	There are 1 total predicted transmembrane domains (TMHMM)
Physical Interactions	There are 35 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI0000129754 \| P14020 MIPS: YPR183W NCBI: 118823 \| 171418 \| 6325441 \| 786315 \| NP_015509.1 GenBank/EMBL/DDBJ: DAA11599.1 \| J04184 \| U25842
External Classifications	EC: 2.4.1.83 [Dolichyl-phosphate beta-D-mannosyltransferase]

Amino Acid Sequence (or in FASTA format)
1 MSIEYSVIVP AYHEKLNIKP LTTRLFAGMS PEMAKKTELI FVDDNSQDGS 51 VEEVDALAHQ GYNVRIIVRT NERGLSSAVL KGFYEAKGQY LVCMDADLQH 101 PPETVPKLFE SLHDHAFTLG TRYAPGVGID KDWPMYRRVI SSTARMMARP 151 LTIASDPMSG FFGLQKKYLE NCNPRDINSQ GFKIALELLA KLPLPRDPRV 201 AIGEVPFTFG VRTEGESKLS GKVIIQYLQQ LKELYVFKFG ANNLILFITF 251 WSILFFYVCY QLYHLVF*

Amino Acid Sequence (or in FASTA format)

       1  MSIEYSVIVP AYHEKLNIKP LTTRLFAGMS PEMAKKTELI FVDDNSQDGS
      51  VEEVDALAHQ GYNVRIIVRT NERGLSSAVL KGFYEAKGQY LVCMDADLQH
     101  PPETVPKLFE SLHDHAFTLG TRYAPGVGID KDWPMYRRVI SSTARMMARP
     151  LTIASDPMSG FFGLQKKYLE NCNPRDINSQ GFKIALELLA KLPLPRDPRV
     201  AIGEVPFTFG VRTEGESKLS GKVIIQYLQQ LKELYVFKFG ANNLILFITF
     251  WSILFFYVCY QLYHLVF*

external links for Dpm1p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
YGOB	BioPIXIE	MIPS	YeastRC Public Image Repository
YOGY	CYC2008 (complexes)	Pfam domains
	Complexome	YeastRC Structure Prediction (Seattle)
	DIP
	GeneMANIA
	YeastRC Mass Spec (Seattle)

References cited on this page View Complete Literature Guide for Dpm1p

1)	Orlean P, et al. (1988) Cloning and sequencing of the yeast gene for dolichol phosphate mannose synthase, an essential protein. J Biol Chem 263(33):17499-507
2)	Orlean P (1990) Dolichol phosphate mannose synthase is required in vivo for glycosyl phosphatidylinositol membrane anchoring, O mannosylation, and N glycosylation of protein in Saccharomyces cerevisiae. Mol Cell Biol 10(11):5796-805
3)	Albright CF, et al. (1989) A 13-amino acid peptide in three yeast glycosyltransferases may be involved in dolichol recognition. Proc Natl Acad Sci U S A 86(19):7366-9
4)	Haselbeck A (1989) Purification of GDP mannose:dolichyl-phosphate O-beta-D-mannosyltransferase from Saccharomyces cerevisiae. Eur J Biochem 181(3):663-8
5)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41