AOS1/YPR180W Protein Information

Standard Name	Aos1p
Systematic Name	Ypr180wp
Alias	Rhc31p 1
ORF Classification	Verified
Description	Subunit of a heterodimeric nuclear SUMO activating enzyme (E1) with Uba2p; activates Smt3p (SUMO) before its conjugation to proteins (sumoylation), which may play a role in protein targeting; essential for viability (2, 3)
Name Description	Activation Of Smt3p 2

Experimental Data
Molecules/cell	7430 4

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	347
Molecular Weight (Da)	39,273
Isoelectric Point (pI)	4.85

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Aos1p (InterPro)
Physical Interactions	There are 23 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI000005319C \| Q06624 MIPS: YPR180W NCBI: 2501437 \| 6325438 \| 786317 \| NP_015506.1 GenBank/EMBL/DDBJ: DAA11596.1 \| U25842

Amino Acid Sequence (or in FASTA format)
1 MDMKVEKLSE DEIALYDRQI RLWGMTAQAN MRSAKVLLIN LGAIGSEITK 51 SIVLSGIGHL TILDGHMVTE EDLGSQFFIG SEDVGQWKID ATKERIQDLN 101 PRIELNFDKQ DLQEKDEEFF QQFDLVVATE MQIDEAIKIN TLTRKLNIPL 151 YVAGSNGLFA YVFIDLIEFI SEDEKLQSVR PTTVGPISSN RSIIEVTTRK 201 DEEDEKKTYE RIKTKNCYRP LNEVLSTATL KEKMTQRQLK RVTSILPLTL 251 SLLQYGLNQK GKAISFEQMK RDAAVWCENL GVPATVVKDD YIQQFIKQKG 301 IEFAPVAAII GGAVAQDVIN ILGKRLSPLN NFIVFDGITL DMPLFEF*

Amino Acid Sequence (or in FASTA format)

       1  MDMKVEKLSE DEIALYDRQI RLWGMTAQAN MRSAKVLLIN LGAIGSEITK
      51  SIVLSGIGHL TILDGHMVTE EDLGSQFFIG SEDVGQWKID ATKERIQDLN
     101  PRIELNFDKQ DLQEKDEEFF QQFDLVVATE MQIDEAIKIN TLTRKLNIPL
     151  YVAGSNGLFA YVFIDLIEFI SEDEKLQSVR PTTVGPISSN RSIIEVTTRK
     201  DEEDEKKTYE RIKTKNCYRP LNEVLSTATL KEKMTQRQLK RVTSILPLTL
     251  SLLQYGLNQK GKAISFEQMK RDAAVWCENL GVPATVVKDD YIQQFIKQKG
     301  IEFAPVAAII GGAVAQDVIN ILGKRLSPLN NFIVFDGITL DMPLFEF*

external links for Aos1p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
Aspergillus (AspGD)	BioPIXIE	MIPS	YeastRC Public Image Repository
Candida (CGD)	CYC2008 (complexes)	Pfam domains
Candida (CandidaDB)	Complexome	YeastRC Structure Prediction (Seattle)
YGOB	DIP
YOGY	GeneMANIA
	YeastRC Two-Hybrid (Seattle)

References cited on this page View Complete Literature Guide for Aos1p

1)	Shayeghi M, et al. (1997) Characterisation of Schizosaccharomyces pombe rad31, a UBA-related gene required for DNA damage tolerance. Nucleic Acids Res 25(6):1162-9
2)	Johnson ES, et al. (1997) The ubiquitin-like protein Smt3p is activated for conjugation to other proteins by an Aos1p/Uba2p heterodimer. EMBO J 16(18):5509-19
3)	Huh WK, et al. (2003) Global analysis of protein localization in budding yeast. Nature 425(6959):686-91
4)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41