VPS4/YPR173C Protein Information

Standard Name	Vps4p 1, 2
Systematic Name	Ypr173cp
Alias	Csc1p 3 , End13p 4, 5 , Grd13p 6 , Vpl4p 7 , Vpt10p 1 , Did6p 8
ORF Classification	Verified
Description	AAA-ATPase involved in multivesicular body (MVB) protein sorting, ATP-bound Vps4p localizes to endosomes and catalyzes ESCRT-III disassembly and membrane release; ATPase activity is activated by Vta1p; regulates cellular sterol metabolism (10, 11, 12, 13, 14, 9)
Name Description	Vacuolar Protein Sorting 1, 2

Experimental Data
Molecules/cell	5350 15

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	437
Molecular Weight (Da)	48,172
Isoelectric Point (pI)	4.96

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Vps4p (InterPro)
Physical Interactions	There are 93 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI0000138D24 \| P52917 MIPS: YPR173C NCBI: 1054845 \| 158430364 \| 158430365 \| 158430366 \| 158430367 \| 158431039 \| 168988715 \| 1706647 \| 208435779 \| 208435780 \| 208435781 \| 208435782 \| 308198533 \| 6325431 \| 786322 \| NP_015499.1 \| NM_001184270.1 GenBank/EMBL/DDBJ: DAA11590.1 \| U25842 \| X92680

Amino Acid Sequence (or in FASTA format)
1 MSTGDFLTKG IELVQKAIDL DTATQYEEAY TAYYNGLDYL MLALKYEKNP 51 KSKDLIRAKF TEYLNRAEQL KKHLESEEAN AAKKSPSAGS GSNGGNKKIS 101 QEEGEDNGGE DNKKLRGALS SAILSEKPNV KWEDVAGLEG AKEALKEAVI 151 LPVKFPHLFK GNRKPTSGIL LYGPPGTGKS YLAKAVATEA NSTFFSVSSS 201 DLVSKWMGES EKLVKQLFAM ARENKPSIIF IDEVDALTGT RGEGESEASR 251 RIKTELLVQM NGVGNDSQGV LVLGATNIPW QLDSAIRRRF ERRIYIPLPD 301 LAARTTMFEI NVGDTPCVLT KEDYRTLGAM TEGYSGSDIA VVVKDALMQP 351 IRKIQSATHF KDVSTEDDET RKLTPCSPGD DGAIEMSWTD IEADELKEPD 401 LTIKDFLKAI KSTRPTVNED DLLKQEQFTR DFGQEGN*

Amino Acid Sequence (or in FASTA format)

       1  MSTGDFLTKG IELVQKAIDL DTATQYEEAY TAYYNGLDYL MLALKYEKNP
      51  KSKDLIRAKF TEYLNRAEQL KKHLESEEAN AAKKSPSAGS GSNGGNKKIS
     101  QEEGEDNGGE DNKKLRGALS SAILSEKPNV KWEDVAGLEG AKEALKEAVI
     151  LPVKFPHLFK GNRKPTSGIL LYGPPGTGKS YLAKAVATEA NSTFFSVSSS
     201  DLVSKWMGES EKLVKQLFAM ARENKPSIIF IDEVDALTGT RGEGESEASR
     251  RIKTELLVQM NGVGNDSQGV LVLGATNIPW QLDSAIRRRF ERRIYIPLPD
     301  LAARTTMFEI NVGDTPCVLT KEDYRTLGAM TEGYSGSDIA VVVKDALMQP
     351  IRKIQSATHF KDVSTEDDET RKLTPCSPGD DGAIEMSWTD IEADELKEPD
     401  LTIKDFLKAI KSTRPTVNED DLLKQEQFTR DFGQEGN*

external links for Vps4p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	Organelle DB
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YPL+
Aspergillus (AspGD)	BioPIXIE	MIPS	YeastGFP
Candida (CGD)	CYC2008 (complexes)	Pfam domains	YeastRC Public Image Repository
Candida (CandidaDB)	Complexome	YeastRC Structure Prediction (Seattle)
YGOB	DIP
YOGY	GeneMANIA
	YeastRC Mass Spec (Seattle)
	YeastRC Two-Hybrid (Seattle)

References cited on this page View Complete Literature Guide for Vps4p

1)	Robinson JS, et al. (1988) Protein sorting in Saccharomyces cerevisiae: isolation of mutants defective in the delivery and processing of multiple vacuolar hydrolases. Mol Cell Biol 8(11):4936-48
2)	Rothman JH, et al. (1989) Characterization of genes required for protein sorting and vacuolar function in the yeast Saccharomyces cerevisiae. EMBO J 8(7):2057-65
3)	Shirahama K, et al. (1997) Mutational analysis of Csc1/Vps4p: involvement of endosome in regulation of autophagy in yeast. Cell Struct Funct 22(5):501-9
4)	Munn AL and Riezman H (1994) Endocytosis is required for the growth of vacuolar H(+)-ATPase-defective yeast: identification of six new END genes. J Cell Biol 127(2):373-86
5)	Zahn R, et al. (2001) End13p/Vps4p is required for efficient transport from early to late endosomes in Saccharomyces cerevisiae. J Cell Sci 114(Pt 10):1935-47
6)	Nothwehr SF, et al. (1996) The newly identified yeast GRD genes are required for retention of late-Golgi membrane proteins. Mol Cell Biol 16(6):2700-7
7)	Rothman JH and Stevens TH (1986) Protein sorting in yeast: mutants defective in vacuole biogenesis mislocalize vacuolar proteins into the late secretory pathway. Cell 47(6):1041-51
8)	Amerik AY, et al. (2000) The Doa4 deubiquitinating enzyme is functionally linked to the vacuolar protein-sorting and endocytic pathways. Mol Biol Cell 11(10):3365-80
9)	Babst M, et al. (1998) The Vps4p AAA ATPase regulates membrane association of a Vps protein complex required for normal endosome function. EMBO J 17(11):2982-93
10)	Kumar A, et al. (2002) Subcellular localization of the yeast proteome. Genes Dev 16(6):707-19
11)	Babst M, et al. (2002) Escrt-III: an endosome-associated heterooligomeric protein complex required for mvb sorting. Dev Cell 3(2):271-82
12)	Wang P, et al. (2005) AAA ATPases regulate membrane association of yeast oxysterol binding proteins and sterol metabolism. EMBO J 24(17):2989-99
13)	Lottridge JM, et al. (2006) Vta1p and Vps46p regulate the membrane association and ATPase activity of Vps4p at the yeast multivesicular body. Proc Natl Acad Sci U S A 103(16):6202-7
14)	Azmi I, et al. (2006) Recycling of ESCRTs by the AAA-ATPase Vps4 is regulated by a conserved VSL region in Vta1. J Cell Biol 172(5):705-17
15)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41