NUT2/YPR168W Protein Information Help

Standard Name Nut2p 1
Systematic Name Ypr168wp
Alias Med10p
ORF Classification Verified
Description Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; required for transcriptional activation and has a role in basal transcription; protein abundance increases in response to DNA replication stress (2, 3)
Name Description Negative regulation of URS Two 1
Experimental Data
Molecules/cell 1470 4
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 157
Molecular Weight (Da) 17,908
Isoelectric Point (pI) 4.71

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Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Nut2p (InterPro)
Physical Interactions There are 92 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI0000053157 | Q06213
MIPS: YPR168W
NCBI: 2833205 | 6325426 | 786293 | NP_015494.1
GenBank/EMBL/DDBJ: DAA11585.1 | U25840
Amino Acid Sequence (or in FASTA format)
 
       1  MNGNSTNNEQ LQQELATTQD QVASIIESFV ELGVSIYDFP GTPEATKGMI
      51  TNLQRNVDRL YKLNVRSNDP QSSLSKVDIP LEVVQYIEDG RNPDIYTREF
     101  VEAIRRSNQY QRGKMHGLKQ LRDSLADKIV DEFPELKEPV EDIIKRTSPI
     151  DNVSNTH*

external links for Nut2p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily Organelle DB
Ashbya (AGD) BOND GPMdb (Mass Spec.) YPL+
Aspergillus (AspGD) BioPIXIE MIPS YeastGFP
Candida (CGD) CYC2008 (complexes) Pfam domains YeastRC Public Image Repository
Candida (CandidaDB) Complexome

YGOB DIP

YOGY GeneMANIA


YeastRC Two-Hybrid (Seattle)

References cited on this page View Complete Literature Guide for Nut2p
1) Tabtiang RK and Herskowitz I  (1998) Nuclear proteins Nut1p and Nut2p cooperate to negatively regulate a Swi4p-dependent lacZ reporter gene in Saccharomyces cerevisiae. Mol Cell Biol 18(8):4707-18
2) Gustafsson CM, et al.  (1998) Identification of new mediator subunits in the RNA polymerase II holoenzyme from Saccharomyces cerevisiae. J Biol Chem 273(47):30851-4
3) Tkach JM, et al.  (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76
4) Ghaemmaghami S, et al.  (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41