ORC4/YPR162C Protein Information

Standard Name	Orc4p 1
Systematic Name	Ypr162cp
ORF Classification	Verified
Description	Subunit of the origin recognition complex (ORC); ORC directs DNA replication by binding to replication origins and is also involved in transcriptional silencing; ORC4 has a paralog, RIF2, that arose from the whole genome duplication (2, 3)
Name Description	Origin Recognition Complex 4

Experimental Data
Molecules/cell	2170 5

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	529
Molecular Weight (Da)	60,705
Isoelectric Point (pI)	6.78

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Orc4p (InterPro)
Physical Interactions	There are 65 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI00000531B3 \| P54791 MIPS: YPR162C NCBI: 1065915 \| 1709490 \| 51830536 \| 6325420 \| 786287 \| NP_015488.1 GenBank/EMBL/DDBJ: DAA11579.1 \| AY723871 \| U25840 \| U34862

Amino Acid Sequence (or in FASTA format)
1 MTISEARLSP QVNLLPIKRH SNEEVEETAA ILKKRTIDNE KCKDSDPGFG 51 SLQRRLLQQL YGTLPTDEKI IFTYLQDCQQ EIDRIIKQSI IQKESHSVIL 101 VGPRQSYKTY LLDYELSLLQ QSYKEQFITI RLNGFIHSEQ TAINGIATQL 151 EQQLQKIHGS EEKIDDTSLE TISSGSLTEV FEKILLLLDS TTKTRNEDSG 201 EVDRESITKI TVVFIFDEID TFAGPVRQTL LYNLFDMVEH SRVPVCIFGC 251 TTKLNILEYL EKRVKSRFSQ RVIYMPQIQN LDDMVDAVRN LLTVRSEISP 301 WVSQWNETLE KELSDPRSNL NRHIRMNFET FRSLPTLKNS IIPLVATSKN 351 FGSLCTAIKS CSFLDIYNKN QLSNNLTGRL QSLSDLELAI LISAARVALR 401 AKDGSFNFNL AYAEYEKMIK AINSRIPTVA PTTNVGTGQS TFSIDNTIKL 451 WLKKDVKNVW ENLVQLDFFT EKSAVGLRDN ATAAFYASNY QFQGTMIPFD 501 LRSYQMQIIL QELRRIIPKS NMYYSWTQL*

Amino Acid Sequence (or in FASTA format)

       1  MTISEARLSP QVNLLPIKRH SNEEVEETAA ILKKRTIDNE KCKDSDPGFG
      51  SLQRRLLQQL YGTLPTDEKI IFTYLQDCQQ EIDRIIKQSI IQKESHSVIL
     101  VGPRQSYKTY LLDYELSLLQ QSYKEQFITI RLNGFIHSEQ TAINGIATQL
     151  EQQLQKIHGS EEKIDDTSLE TISSGSLTEV FEKILLLLDS TTKTRNEDSG
     201  EVDRESITKI TVVFIFDEID TFAGPVRQTL LYNLFDMVEH SRVPVCIFGC
     251  TTKLNILEYL EKRVKSRFSQ RVIYMPQIQN LDDMVDAVRN LLTVRSEISP
     301  WVSQWNETLE KELSDPRSNL NRHIRMNFET FRSLPTLKNS IIPLVATSKN
     351  FGSLCTAIKS CSFLDIYNKN QLSNNLTGRL QSLSDLELAI LISAARVALR
     401  AKDGSFNFNL AYAEYEKMIK AINSRIPTVA PTTNVGTGQS TFSIDNTIKL
     451  WLKKDVKNVW ENLVQLDFFT EKSAVGLRDN ATAAFYASNY QFQGTMIPFD
     501  LRSYQMQIIL QELRRIIPKS NMYYSWTQL*

external links for Orc4p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	Organelle DB
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YPL+
Aspergillus (AspGD)	BioPIXIE	MIPS	YeastGFP
Candida (CGD)	CYC2008 (complexes)	Pfam domains
Candida (CandidaDB)	Complexome	YeastRC Structure Prediction (Seattle)
YGOB	DIP
YOGY	GeneMANIA
	YeastRC Two-Hybrid (Seattle)

References cited on this page View Complete Literature Guide for Orc4p

1)	Bell SP, et al. (1995) The multidomain structure of Orc1p reveals similarity to regulators of DNA replication and transcriptional silencing. Cell 83(4):563-8
2)	Bell SP (2002) The origin recognition complex: from simple origins to complex functions. Genes Dev 16(6):659-72
3)	Byrne KP and Wolfe KH (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
4)	Bell SP and Stillman B (1992) ATP-dependent recognition of eukaryotic origins of DNA replication by a multiprotein complex. Nature 357(6374):128-34
5)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41