SGV1/YPR161C Protein Information Help

Standard Name Sgv1p 1
Systematic Name Ypr161cp
Alias Bur1p 2
ORF Classification Verified
Description Cyclin (Bur2p)-dependent protein kinase that functions in transcriptional regulation; phosphorylates the carboxy-terminal domain of Rpo21p and the C-terminal repeat domain of Spt5p; regulated by Cak1p; similar to metazoan CDK9 proteins (2, 3, 4, 5, 6, 7, 8)
Name Description Suppressor of Gpa1Val50 mutation 1
Experimental Data
Molecules/cell 2070 9
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 657
Molecular Weight (Da) 74,239
Isoelectric Point (pI) 10.36

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Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Sgv1p (InterPro)
Physical Interactions There are 111 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI00000530F1 | P23293
MIPS: YPR161C
NCBI: 134474 | 218486 | 499701 | 6325419 | 849169 | NP_015487.1
GenBank/EMBL/DDBJ: DAA11578.1 | D90317 | L33835 | U28371
External Classifications EC: 2.7.11.22 [Cyclin-dependent kinase]
EC: 2.7.11.23 [[RNA-polymerase]-subunit kinase]
Amino Acid Sequence (or in FASTA format)
 
       1  MSDNGSPAVL PKTEFNKYKI GKVKSTPAIQ RDAKTNLTYI KLRKRSSEKV
      51  YGCTVFQNHY REDEKLGQGT FGEVYKGIHL ETQRQVAMKK IIVSVEKDLF
     101  PITAQREITI LKRLNHKNII KLIEMVYDHS PDITNAASSN LHKSFYMILP
     151  YMVADLSGVL HNPRINLEMC DIKNMMLQIL EGLNYIHCAK FMHRDIKTAN
     201  ILIDHNGVLK LADFGLARLY YGCPPNLKYP GGAGSGAKYT SVVVTRWYRA
     251  PELVLGDKQY TTAVDIWGVG CVFAEFFEKK PILQGKTDID QGHVIFKLLG
     301  TPTEEDWAVA RYLPGAELTT TNYKPTLRER FGKYLSETGL DFLGQLLALD
     351  PYKRLTAMSA KHHPWFKEDP LPSEKITLPT EESHEADIKR YKEEMHQSLS
     401  QRVPTAPRGH IVEKGESPVV KNLGAIPRGP KKDDASFLPP SKNVLAKPPP
     451  SKIRELHQNP RPYHVNSGYA KTAIPPPAAP AGVNRYGPNN SSRNNRFSGN
     501  STAPNNSRNP VNRFHPETNV SSKYNKVPLP LGPQSRYQGN SNESRYKNSP
     551  NDSRYHNPRY VNKPETNFNR QPQKYSRQES NAPINKNYNP SNGSRNMAGD
     601  HHQGSRPSHP QFPISPSQGQ HQLTSKPIEK KNGSFKDERA KPDESKEFQN
     651  SDIADLY*

external links for Sgv1p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily Organelle DB
Ashbya (AGD) BOND GPMdb (Mass Spec.) YPL+
YGOB BioPIXIE MIPS YeastGFP
YOGY CYC2008 (complexes) Pfam domains YeastRC Public Image Repository

Complexome YeastRC Structure Prediction (Seattle)

DIP


GeneMANIA

References cited on this page View Complete Literature Guide for Sgv1p
1) Irie K, et al.  (1991) SGV1 encodes a CDC28/cdc2-related kinase required for a G alpha subunit-mediated adaptive response to pheromone in S. cerevisiae. Cell 65(5):785-95
2) Prelich G and Winston F  (1993) Mutations that suppress the deletion of an upstream activating sequence in yeast: involvement of a protein kinase and histone H3 in repressing transcription in vivo. Genetics 135(3):665-76
3) Yao S, et al.  (2000) BUR1 and BUR2 encode a divergent cyclin-dependent kinase-cyclin complex important for transcription in vivo. Mol Cell Biol 20(19):7080-7
4) Murray S, et al.  (2001) Phosphorylation of the RNA polymerase II carboxy-terminal domain by the Bur1 cyclin-dependent kinase. Mol Cell Biol 21(13):4089-96
5) Yao S and Prelich G  (2002) Activation of the Bur1-Bur2 cyclin-dependent kinase complex by Cak1. Mol Cell Biol 22(19):6750-8
6) Keogh MC, et al.  (2003) Bur1 kinase is required for efficient transcription elongation by RNA polymerase II. Mol Cell Biol 23(19):7005-18
7) Liu Y, et al.  (2009) Phosphorylation of the transcription elongation factor Spt5 by yeast Bur1 kinase stimulates recruitment of the PAF complex. Mol Cell Biol 29(17):4852-63
8) Bartkowiak B, et al.  (2010) CDK12 is a transcription elongation-associated CTD kinase, the metazoan ortholog of yeast Ctk1. Genes Dev 24(20):2303-16
9) Ghaemmaghami S, et al.  (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41