TPO3/YPR156C Protein Information Help

Standard Name Tpo3p 1
Systematic Name Ypr156cp
ORF Classification Verified
Description Polyamine transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; specific for spermine; localizes to the plasma membrane; TPO3 has a paralog, TPO2, that arose from the whole genome duplication (1, 2, 3, 4)
Name Description Transporter of POlyamines 5
Experimental Data
Molecules/cell 2760 6
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 622
Molecular Weight (Da) 68,065
Isoelectric Point (pI) 7.04

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Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Tpo3p (InterPro)
Transmembrane Domains There are 12 total predicted transmembrane domains (TMHMM)
Physical Interactions There are 57 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI000006AD66 | Q06451
MIPS: YPR156C
NCBI: 51830534 | 6325414 | 74676396 | 849164 | NP_015482.1 | NM_001184253.1
GenBank/EMBL/DDBJ: AAB68053.1 | AAU09787.1 | DAA11568.1 | AY723870 | U28371
External Classifications TC: 2.A.1.2 [The Drug:H+ Antiporter-1 (12 Spanner) (DHA1) Family]
Amino Acid Sequence (or in FASTA format)
       1  MNRQESINSF NSDETSSLSD VESQQPQQYI PSESGSKSNM APNQLKLTRT
      51  ETVKSLQDMG VSSKAPVPDV NAPQSSKNKI FPEEYTLETP TGLVPVATLH
     101  SIGRTSTAIS RTRTRQIDGA SSPSSNEDAL ESDNNEKGKE GDSSGANDEA
     151  PDLDPEIEFV TFVTGDPENP HNWPAWIRWS YTVLLSILVI CVAYGSACIS
     201  GGLGTVEKKY HVGMEAAILS VSLMVIGFSL GPLIWSPVSD LYGRRVAYFV
     251  SMGLYVIFNI PCALAPNLGS LLACRFLCGV WSSSGLCLVG GSIADMFPSE
     301  TRGKAIAFFA FAPYVGPVVG PLVNGFISVS TGRMDLIFWV NMAFAGVMWI
     351  ISSAIPETYA PVILKRKAAR LRKETGNPKI MTEQEAQGVS MGEMMRACLL
     401  RPLYFSVTEP VLVATCFYVC LIYSLLYAFF FAFPVIFGEL YGYKDNLVGL
     451  MFIPIVIGAL WALATTFYCE NKYLQIVKQR KPTPEDRLLG AKIGAPFAAI
     501  ALWILGATAY KHIIWVGPAS AGLAFGFGMV LIYYSLNNYI IDCYVQYASS
     551  ALATKVFLRS AGGAAFPLFT IQMYHKLNLH WGSWLLAFIS TAMIALPFAF
     601  SYWGKGLRHK LSKKDYSIDS IE*                             

external links for Tpo3p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily LoQate
Ashbya (AGD) BOND GPMdb (Mass Spec.) YPL+
YGOB CYC2008 (complexes) MIPS YeastGFP
YOGY Complexome Pfam domains YeastRC Public Image Repository

DIP TCDB

GeneMANIA YeastRC Structure Prediction (Seattle)

IMP

References cited on this page View Complete Literature Guide for Tpo3p
1) Tomitori H, et al.  (2001) Multiple polyamine transport systems on the vacuolar membrane in yeast. Biochem J 353(Pt 3):681-8
2) Albertsen M, et al.  (2003) Localization and function of the yeast multidrug transporter Tpo1p. J Biol Chem 278(15):12820-5
3) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
4) Dias PJ, et al.  (2010) Evolution of the 12-Spanner Drug:H+ Antiporter DHA1 Family in Hemiascomycetous Yeasts. OMICS 14(6):701-10
5) Tomitori H, et al.  (1999) Identification of a gene for a polyamine transport protein in yeast. J Biol Chem 274(6):3265-7
6) Ghaemmaghami S, et al.  (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41