ASN1/YPR145W Protein Information

Standard Name	Asn1p 1, 2
Systematic Name	Ypr145wp
ORF Classification	Verified
Description	Asparagine synthetase; catalyzes the synthesis of L-asparagine from L-aspartate in the asparagine biosynthetic pathway; ASN1 has a paralog, ASN2, that arose from the whole genome duplication (2, 3)
Name Description	ASparagiNe requiring 1

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	572
Molecular Weight (Da)	64,470
Isoelectric Point (pI)	6.04

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Asn1p (InterPro)
Physical Interactions	There are 14 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI000016843C \| P49089 MIPS: YPR145W NCBI: 1066479 \| 1351984 \| 6325403 \| 747902 \| NP_015471.1 GenBank/EMBL/DDBJ: DAA11558.1 \| U40829 \| Z48675
External Classifications	EC: 6.3.5.4 [Asparagine synthase (glutamine-hydrolyzing)]

Amino Acid Sequence (or in FASTA format)
1 MCGIFAAFRH EDVHRYKPKA LQLSKRIRHR GPDWSGNAIK NSTIFVHERL 51 AIVGVESGAQ PITSSDGEYM LCVNGEIYNH IQLREECADY EFGTLSDCEP 101 IIPMYLKHDI DAPKYLDGMF AWTLYDAKQD RIVAARDPIG ITTLYMGRSS 151 ASPKTVYFAS ELKCLTDDCD TITAFPPGHV YDSKTDKITR YFTPDWLDEK 201 RIPSTPIDYM AIRHSLEKAV RKRLMAEVPY GVLLSGGLDS SLIASIAARE 251 TAKATNDVEP STYDSKARHL AGIDDDGKLH TAGWTSLHSF AIGLPNAPDL 301 QAARKVAKFI GSIHHEHTFT LQEGLDALDD VIYHLETYDV TTIRASTPMF 351 LLSRKIKAQG VKMVLSGEGS DEIFGGYLYF AQAPSAAEFH TESVQRVKNL 401 HLADCLRANK STMAWGLEAR VPFLDREFLQ LCMNIDPNEK MIKPKEGRIE 451 KYILRKAFDT TGEPDAKPYL PEEILWRQKE QFSDGVGYSW IDGLKDTAEA 501 VISDEMFASP KAEWGSDIPT TKEAFWYRLK FDALFPQKTV ADTVMRWIPK 551 ADWGCAEDPS GRYAQIHEKH IE*

Amino Acid Sequence (or in FASTA format)

       1  MCGIFAAFRH EDVHRYKPKA LQLSKRIRHR GPDWSGNAIK NSTIFVHERL
      51  AIVGVESGAQ PITSSDGEYM LCVNGEIYNH IQLREECADY EFGTLSDCEP
     101  IIPMYLKHDI DAPKYLDGMF AWTLYDAKQD RIVAARDPIG ITTLYMGRSS
     151  ASPKTVYFAS ELKCLTDDCD TITAFPPGHV YDSKTDKITR YFTPDWLDEK
     201  RIPSTPIDYM AIRHSLEKAV RKRLMAEVPY GVLLSGGLDS SLIASIAARE
     251  TAKATNDVEP STYDSKARHL AGIDDDGKLH TAGWTSLHSF AIGLPNAPDL
     301  QAARKVAKFI GSIHHEHTFT LQEGLDALDD VIYHLETYDV TTIRASTPMF
     351  LLSRKIKAQG VKMVLSGEGS DEIFGGYLYF AQAPSAAEFH TESVQRVKNL
     401  HLADCLRANK STMAWGLEAR VPFLDREFLQ LCMNIDPNEK MIKPKEGRIE
     451  KYILRKAFDT TGEPDAKPYL PEEILWRQKE QFSDGVGYSW IDGLKDTAEA
     501  VISDEMFASP KAEWGSDIPT TKEAFWYRLK FDALFPQKTV ADTVMRWIPK
     551  ADWGCAEDPS GRYAQIHEKH IE*

external links for Asn1p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	Organelle DB
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YPL+
YGOB	BioPIXIE	MIPS	YeastGFP
YOGY	CYC2008 (complexes)	Pfam domains	YeastRC Public Image Repository
	Complexome	YeastRC Structure Prediction (Seattle)
	DIP
	GeneMANIA

References cited on this page View Complete Literature Guide for Asn1p

1)	Jones GE (1978) L-Asparagine auxotrophs of Saccharomyces cerevisiae: genetic and phenotypic characterization. J Bacteriol 134(1):200-7
2)	Dang VD, et al. (1996) Cloning of the ASN1 and ASN2 genes encoding asparagine synthetases in Saccharomyces cerevisiae: differential regulation by the CCAAT-box-binding factor. Mol Microbiol 22(4):681-92
3)	Byrne KP and Wolfe KH (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61