KAR3/YPR141C Protein Information Help

Standard Name Kar3p
Systematic Name Ypr141cp
Alias Osr11p
ORF Classification Verified
Description Minus-end-directed microtubule motor that functions in mitosis and meiosis, localizes to the spindle pole body and localization is dependent on functional Cik1p, required for nuclear fusion during mating; potential Cdc28p substrate (1, 2, 3, 4, 5)
Name Description KARyogamy
Experimental Data
Molecules/cell 3250 6
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 729
Molecular Weight (Da) 84,003
Isoelectric Point (pI) 9.39

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Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Kar3p (InterPro)
Physical Interactions There are 70 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI000012DC35 | P17119
MIPS: YPR141C
NCBI: 1066492 | 125224 | 14719772 | 14719773 | 14719774 | 14719775 | 14719776 | 157836831 | 171775 | 392935685 | 6325399 | NP_015467.1
GenBank/EMBL/DDBJ: DAA11555.1 | M31719 | U40829
Amino Acid Sequence (or in FASTA format)
 
       1  MESLPRTPTK GRSTQHLSTP SPKNDILAMN GHKRRNTTTP PPKHTLLKPQ
      51  RTDIHRHSLA SQSRISMSPN RELLKNYKGT ANLIYGNQKS NSGVTSFYKE
     101  NVNELNRTQA ILFEKKATLD LLKDELTETK EKINAVNLKF ETLREEKIKI
     151  EQQLNLKNNE LISIKEEFLS KKQFMNEGHE IHLKQLAASN KKELKQMENE
     201  YKTKIEKLKF MKIKQFENER ASLLDKIEEV RNKITMNPST LQEMLNDVEQ
     251  KHMLEKEEWL TEYQSQWKKD IELNNKHMQE IESIKKEIEN TLKPELAEKK
     301  KLLTEKRNAY EAIKVKVKEK EEETTRLRDE VALKQKTNLE TLEKIKELEE
     351  YIKDTELGMK ELNEILIKEE TVRRTLHNEL QELRGNIRVY CRIRPALKNL
     401  ENSDTSLINV NEFDDNSGVQ SMEVTKIQNT AQVHEFKFDK IFDQQDTNVD
     451  VFKEVGQLVQ SSLDGYNVCI FAYGQTGSGK TFTMLNPGDG IIPSTISHIF
     501  NWINKLKTKG WDYKVNCEFI EIYNENIVDL LRSDNNNKED TSIGLKHEIR
     551  HDQETKTTTI TNVTSCKLES EEMVEIILKK ANKLRSTAST ASNEHSSRSH
     601  SIFIIHLSGS NAKTGAHSYG TLNLVDLAGS ERINVSQVVG DRLRETQNIN
     651  KSLSCLGDVI HALGQPDSTK RHIPFRNSKL TYLLQYSLTG DSKTLMFVNI
     701  SPSSSHINET LNSLRFASKV NSTRLVSRK*

external links for Kar3p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily YPL+
Ashbya (AGD) BOND GPMdb (Mass Spec.) YeastGFP
Candida (CGD) BioPIXIE MIPS YeastRC Public Image Repository
YGOB CYC2008 (complexes) Pfam domains
YOGY Complexome YeastRC Structure Prediction (Seattle)

DIP


GeneMANIA


YeastRC Two-Hybrid (Seattle)

References cited on this page View Complete Literature Guide for Kar3p
1) Meluh PB and Rose MD  (1990) KAR3, a kinesin-related gene required for yeast nuclear fusion. Cell 60(6):1029-41
2) Page BD, et al.  (1994) Localization of the Kar3 kinesin heavy chain-related protein requires the Cik1 interacting protein. J Cell Biol 124(4):507-19
3) Middleton K and Carbon J  (1994) KAR3-encoded kinesin is a minus-end-directed motor that functions with centromere binding proteins (CBF3) on an in vitro yeast kinetochore. Proc Natl Acad Sci U S A 91(15):7212-6
4) Mackey AT and Gilbert SP  (2003) The ATPase cross-bridge cycle of the Kar3 motor domain. Implications for single head motility. J Biol Chem 278(6):3527-35
5) Ubersax JA, et al.  (2003) Targets of the cyclin-dependent kinase Cdk1. Nature 425(6960):859-64
6) Ghaemmaghami S, et al.  (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41