NAT3/YPR131C Protein Information

Standard Name	Nat3p 1
Systematic Name	Ypr131cp
Alias	Naa20p 2 , Rad56p 3
ORF Classification	Verified
Description	Catalytic subunit of the NatB N-terminal acetyltransferase, which catalyzes acetylation of the amino-terminal methionine residues of all proteins beginning with Met-Asp or Met-Glu and of some proteins beginning with Met-Asn or Met-Met (4, 5)
Name Description	N-terminal AcetylTransferase 6

Experimental Data
Molecules/cell	639 7

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	195
Molecular Weight (Da)	22,921
Isoelectric Point (pI)	7.52

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Nat3p (InterPro)
Physical Interactions	There are 14 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI000019C42C \| Q06504 MIPS: YPR131C NCBI: 1066488 \| 31126970 \| 83288310 \| NP_015456.2 \| NM_001184228.1 GenBank/EMBL/DDBJ: DAA11543.1 \| U40829
External Classifications	EC: 2.3.1.88 [Peptide alpha-N-acetyltransferase]

Amino Acid Sequence (or in FASTA format)
1 MTTIQPFEPV DLFKTNNVNL DILTENFPLE FYFEYMIIWP DLFFKSSEMT 51 VDPTFKHNIS GYMMAKTEGK TTEWHTHITA VTVAPRFRRI SLASKLCNTL 101 ETMTDVMPHE VNFIDLFVKC NNQLAIKLYE KLGYSVYRRV VGYYNSAEDG 151 YPDTLKKVDD NKDAFDMRKA MARDRNRSVR PDGRSHKCYP HDVRF*

Amino Acid Sequence (or in FASTA format)

       1  MTTIQPFEPV DLFKTNNVNL DILTENFPLE FYFEYMIIWP DLFFKSSEMT
      51  VDPTFKHNIS GYMMAKTEGK TTEWHTHITA VTVAPRFRRI SLASKLCNTL
     101  ETMTDVMPHE VNFIDLFVKC NNQLAIKLYE KLGYSVYRRV VGYYNSAEDG
     151  YPDTLKKVDD NKDAFDMRKA MARDRNRSVR PDGRSHKCYP HDVRF*

external links for Nat3p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	Organelle DB
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YPL+
Candida (CGD)	BioPIXIE	MIPS	YeastGFP
Candida (CandidaDB)	CYC2008 (complexes)	Pfam domains
YGOB	Complexome	YeastRC Structure Prediction (Seattle)
YOGY	GeneMANIA

References cited on this page View Complete Literature Guide for Nat3p

1)	Polevoda B, et al. (1999) Identification and specificities of N-terminal acetyltransferases from Saccharomyces cerevisiae. EMBO J 18(21):6155-68
2)	Polevoda B, et al. (2009) A synopsis of eukaryotic Nalpha-terminal acetyltransferases: nomenclature, subunits and substrates. BMC Proc 3 Suppl 6:S2
3)	Mathiasen DP, et al. (2013) Physical mapping and cloning of RAD56. Gene 519(1):182-6
4)	Singer JM and Shaw JM (2003) Mdm20 protein functions with Nat3 protein to acetylate Tpm1 protein and regulate tropomyosin-actin interactions in budding yeast. Proc Natl Acad Sci U S A 100(13):7644-9
5)	Polevoda B and Sherman F (2003) Composition and function of the eukaryotic N-terminal acetyltransferase subunits. Biochem Biophys Res Commun 308(1):1-11
6)	Mullen JR, et al. (1989) Identification and characterization of genes and mutants for an N-terminal acetyltransferase from yeast. EMBO J 8(7):2067-75
7)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41