DBF20/YPR111W Protein Information Help

Standard Name Dbf20p 1
Systematic Name Ypr111wp
ORF Classification Verified
Description Ser/Thr kinase involved in late nuclear division; one of the mitotic exit network (MEN) proteins; necessary for the execution of cytokinesis; also plays a role in regulating the stability of SWI5 and CLB2 mRNAs; DBF20 has a paralog, DBF2, that arose from the whole genome duplication (2, 3, 4, 5, 6)
Name Description DumbBell Forming 7
Experimental Data
Molecules/cell 3320 8
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 564
Molecular Weight (Da) 65,879
Isoelectric Point (pI) 8.86

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Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Dbf20p (InterPro)
Physical Interactions There are 16 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI0000128EE8 | P32328
MIPS: YPR111W
NCBI: 171380 | 2347150 | 3123195 | 51013161 | 6325368 | NP_015436.1
GenBank/EMBL/DDBJ: DAA11526.1 | AY692855 | M62506 | U32445
External Classifications EC: 2.7.11.1 [Non-specific serine/threonine protein kinase]
Amino Acid Sequence (or in FASTA format)
 
       1  MFSRSDREVD DLAGNMSHLG FYDLNIPKPT SPQAQYRPAR KSENGRLTPG
      51  LPRSYKPCDS DDQDTFKNRI SLNHSPKKLP KDFHERASQS KTQRVVNVCQ
     101  LYFLDYYCDM FDYVISRRQR TKQVLRYLEQ QRSVKNVSNK VLNEEWALYL
     151  QREHEVLRKR RLKPKHKDFQ ILTQVGQGGY GQVYLAKKKD SDEICALKIL
     201  NKKLLFKLNE TNHVLTERDI LTTTRSDWLV KLLYAFQDPE SLYLAMEFVP
     251  GGDFRTLLIN TRILKSGHAR FYISEMFCAV NALHELGYTH RDLKPENFLI
     301  DATGHIKLTD FGLAAGTVSN ERIESMKIRL EEVKNLEFPA FTERSIEDRR
     351  KIYHNMRKTE INYANSMVGS PDYMALEVLE GKKYDFTVDY WSLGCMLFES
     401  LVGYTPFSGS STNETYENLR YWKKTLRRPR TEDRRAAFSD RTWDLITRLI
     451  ADPINRVRSF EQVRKMSYFA EINFETLRTS SPPFIPQLDD ETDAGYFDDF
     501  TNEEDMAKYA DVFKRQNKLS AMVDDSAVDS KLVGFTFRHR DGKQGSSGIL
     551  YNGSEHSDPF STFY*

external links for Dbf20p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily YPL+
Ashbya (AGD) BOND GPMdb (Mass Spec.) YeastGFP
Aspergillus (AspGD) BioPIXIE MIPS YeastRC Public Image Repository
Candida (CGD) CYC2008 (complexes) Pfam domains
Candida (CandidaDB) Complexome

YGOB DIP

YOGY GeneMANIA

References cited on this page View Complete Literature Guide for Dbf20p
1) Johnston, L.  (1992) Personal Communication, Mortimer Map Edition 11
2) Toyn JH and Johnston LH  (1994) The Dbf2 and Dbf20 protein kinases of budding yeast are activated after the metaphase to anaphase cell cycle transition. EMBO J 13(5):1103-13
3) Menssen R, et al.  (2001) Asymmetric spindle pole localization of yeast Cdc15 kinase links mitotic exit and cytokinesis. Curr Biol 11(5):345-50
4) Hwa Lim H, et al.  (2003) Inactivation of mitotic kinase triggers translocation of MEN components to mother-daughter neck in yeast. Mol Biol Cell 14(11):4734-43
5) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
6) Trcek T, et al.  (2011) Single-molecule mRNA decay measurements reveal promoter- regulated mRNA stability in yeast. Cell 147(7):1484-97
7) Johnston LH and Thomas AP  (1982) The isolation of new DNA synthesis mutants in the yeast Saccharomyces cerevisiae. Mol Gen Genet 186(3):439-44
8) Ghaemmaghami S, et al.  (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41