FHL1/YPR104C Protein Information

Standard Name	Fhl1p 1
Systematic Name	Ypr104cp
Alias	Spp42p 2, 3
ORF Classification	Verified
Description	Regulator of ribosomal protein (RP) transcription; has forkhead associated domain that binds phosphorylated proteins; recruits coactivator Ifh1p or corepressor Crf1p to RP gene promoters; also has forkhead DNA-binding domain though in vitro DNA binding assays give inconsistent results; computational analyses suggest it binds DNA directly at highly active RP genes and indirectly through Rap1p motifs at others; suppresses RNA pol III and splicing factor prp4 mutants (1, 2, 4, 5, 6, 7, 8, 9)
Name Description	Fork Head-Like 1

Experimental Data
Molecules/cell	639 10

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	936
Molecular Weight (Da)	103,501
Isoelectric Point (pI)	5.7

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Fhl1p (InterPro)
Physical Interactions	There are 21 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI000012A734 \| P39521 MIPS: YPR104C NCBI: 454255 \| 6325361 \| 729486 \| 914975 \| NP_015429.1 GenBank/EMBL/DDBJ: DAA11518.1 \| U32445 \| Z28348

Amino Acid Sequence (or in FASTA format)
1 MDGEMAIIES SNHVGTSSPT TETQFTIDSS ALKDQETKES ITNSPTSEVP 51 IETKLPKSSD IVTEEKHPQN TTTDIENEVE NPVTDDNGNL KLELPDNLDN 101 ADFSKLLEFD AKNDEALFNS NELLSHTMDP VNNIDLTHDH SREVSSKEDI 151 NIEPVNPDED EREKTQDNTA AVKTEGIRNS EDTSIQKDEP TADAIYTDVH 201 KLSVNKDTET LPTLVDEKNN MLHMRNNSIT PIMFQQHELV GQPPQNTVTE 251 NNSTDAETTQ RKLSEPIDAS LPLPNEQPTI FAYARLDFQS FTFYVQTLHA 301 IIGRRSENDF SHKVDVNLGP SKSISRRHAQ IFYNFGTGRF ELSIIGKNGA 351 FVDDIFVEKG NTVPLRNKTK IQIGQIPFQF ILPEQERNDD SKSPENADIA 401 ESEINTRNLK KNEPKSKKKI TTGAKPKKAQ TKPAVKKEKK PPKIPKKVYT 451 LEEIPVEYRT KPTVSYSAML TTCIRKYSTA KGMSLSEIYA GIRELFPYYK 501 YCPDGWQSSV RHNLSLNKSF RKVSKEGKGW LWGLDEEYIA ERERQKKKQS 551 EIAVAKAQAA QLKLEQQQHK LQQVPQRGKK DIVSQRSNVN ARKQNISQTL 601 AANRAASNRK NTASDNQRTM KYLQEQLVIL TRDRKGLSKQ VIAAILTQAL 651 AMTINQVTQA AKNKGITGNP LTALMDKNPQ HLNLILAAAV NAATAKVTKG 701 EVKQLVNPET TAAAALAAKA QHSKPIRQPI VQTPHVPDRP PSQLSASASS 751 HPNNYLHDKQ PGSFDPSSLS RFFQPRQNAR ATSSVAATSV PAAASQNVDA 801 QPKPKPAQDN DLESESGTSS SSSSSSESGS ESDSGSDDGS ASGSGDNSST 851 SSESESESDS GSEVDEKNNK NEKIDSESIK NNESKDDIPS KDENSSNDNR 901 EISKTDEEGH DSKRRKVSED INEGITEVNV SLEEKL*

Amino Acid Sequence (or in FASTA format)

       1  MDGEMAIIES SNHVGTSSPT TETQFTIDSS ALKDQETKES ITNSPTSEVP
      51  IETKLPKSSD IVTEEKHPQN TTTDIENEVE NPVTDDNGNL KLELPDNLDN
     101  ADFSKLLEFD AKNDEALFNS NELLSHTMDP VNNIDLTHDH SREVSSKEDI
     151  NIEPVNPDED EREKTQDNTA AVKTEGIRNS EDTSIQKDEP TADAIYTDVH
     201  KLSVNKDTET LPTLVDEKNN MLHMRNNSIT PIMFQQHELV GQPPQNTVTE
     251  NNSTDAETTQ RKLSEPIDAS LPLPNEQPTI FAYARLDFQS FTFYVQTLHA
     301  IIGRRSENDF SHKVDVNLGP SKSISRRHAQ IFYNFGTGRF ELSIIGKNGA
     351  FVDDIFVEKG NTVPLRNKTK IQIGQIPFQF ILPEQERNDD SKSPENADIA
     401  ESEINTRNLK KNEPKSKKKI TTGAKPKKAQ TKPAVKKEKK PPKIPKKVYT
     451  LEEIPVEYRT KPTVSYSAML TTCIRKYSTA KGMSLSEIYA GIRELFPYYK
     501  YCPDGWQSSV RHNLSLNKSF RKVSKEGKGW LWGLDEEYIA ERERQKKKQS
     551  EIAVAKAQAA QLKLEQQQHK LQQVPQRGKK DIVSQRSNVN ARKQNISQTL
     601  AANRAASNRK NTASDNQRTM KYLQEQLVIL TRDRKGLSKQ VIAAILTQAL
     651  AMTINQVTQA AKNKGITGNP LTALMDKNPQ HLNLILAAAV NAATAKVTKG
     701  EVKQLVNPET TAAAALAAKA QHSKPIRQPI VQTPHVPDRP PSQLSASASS
     751  HPNNYLHDKQ PGSFDPSSLS RFFQPRQNAR ATSSVAATSV PAAASQNVDA
     801  QPKPKPAQDN DLESESGTSS SSSSSSESGS ESDSGSDDGS ASGSGDNSST
     851  SSESESESDS GSEVDEKNNK NEKIDSESIK NNESKDDIPS KDENSSNDNR
     901  EISKTDEEGH DSKRRKVSED INEGITEVNV SLEEKL*

external links for Fhl1p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	Organelle DB
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YPL+
YGOB	BioPIXIE	MIPS	YeastGFP
YOGY	CYC2008 (complexes)	Pfam domains	YeastRC Public Image Repository
	Complexome	YeastRC Structure Prediction (Seattle)
	DIP
	GeneMANIA

References cited on this page View Complete Literature Guide for Fhl1p

1)	Hermann-Le Denmat S, et al. (1994) Suppression of yeast RNA polymerase III mutations by FHL1, a gene coding for a fork head protein involved in rRNA processing. Mol Cell Biol 14(5):2905-13
2)	Maddock JR, et al. (1994) Extragenic suppressors of Saccharomyces cerevisiae prp4 mutations identify a negative regulator of PRP genes. Genetics 136(3):833-47
3)	Maddock J (1990) Genetic interactions among yeast gene products required for messenger RNA processing. Ph.D. thesis, Carnegie Mellon University, United States
4)	Martin DE, et al. (2004) TOR regulates ribosomal protein gene expression via PKA and the Forkhead transcription factor FHL1. Cell 119(7):969-79
5)	Rudra D, et al. (2005) Central role of Ifh1p-Fhl1p interaction in the synthesis of yeast ribosomal proteins. EMBO J 24(3):533-42
6)	Badis G, et al. (2008) A library of yeast transcription factor motifs reveals a widespread function for Rsc3 in targeting nucleosome exclusion at promoters. Mol Cell 32(6):878-87
7)	Zhu C, et al. (2009) High-resolution DNA-binding specificity analysis of yeast transcription factors. Genome Res 19(4):556-66
8)	Xiao L and Grove A (2009) Coordination of Ribosomal Protein and Ribosomal RNA Gene Expression in Response to TOR Signaling. Curr Genomics 10(3):198-205
9)	Zeevi D, et al. (2011) Compensation for differences in gene copy number among yeast ribosomal proteins is encoded within their promoters. Genome Res 21(12):2114-28
10)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41