MED1/YPR070W Protein Information

Standard Name	Med1p
Systematic Name	Ypr070wp
ORF Classification	Verified
Description	Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation (1, 2)
Name Description	MEDiator complex

Experimental Data
Molecules/cell	4670 3

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	566
Molecular Weight (Da)	64,251
Isoelectric Point (pI)	4.52

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Med1p (InterPro)
Physical Interactions	There are 132 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI0000053150 \| Q12321 MIPS: YPR070W NCBI: 1230678 \| 1314138 \| 6325327 \| 74676582 \| 805050 \| NP_015395.1 GenBank/EMBL/DDBJ: DAA11490.1 \| U51033 \| Z49219 \| Z71255

Amino Acid Sequence (or in FASTA format)
1 MVEGDSYVET LDSMIELFKD YKPGSITLEN ITRLCQTLGL ESFTEELSNE 51 LSRLSTASKI IVIDVDYNKK QDRIQDVKLV LASNFDNFDY FNQRDGEHEK 101 SNILLNSLTK YPDLKAFHNN LKFLYLLDAY SHIESDSTSH NNGSSDKSLD 151 SSNASFNNQG KLDLFKYFTE LSHYIRQCFQ DNCCDFKVRT NLNDKFGIYI 201 LTQGINGKEV PLAKIYLEEN KSDSQYRFYE YIYSQETKSW INESAENFSN 251 GISLVMEIVA NAKESNYTDL IWFPEDFISP ELIIDKVTCS SNSSSSPPII 301 DLFSNNNYNS RIQLMNDFTT KLINIKKFDI SNDNLDLISE ILKWVQWSRI 351 VLQNVFKLVS TPSSNSNSSE LEPDYQAPFS TSTKDKNSST SNTEPIPRSN 401 RHGSVVEASR RRRSSTNKSK RPSITEAMML KEEGLQQFNL HEILSEPAIE 451 EENGDSIKEH STTMDGANDL GFTASVSNQE NAGTDIVMED HGVLQGTSQN 501 YGTATADDAD IEMKDVSSKP SKPESSVLQL IVSEDHIILD TISECNLYDD 551 VKCWSKFIEK FQDIVS*

Amino Acid Sequence (or in FASTA format)

       1  MVEGDSYVET LDSMIELFKD YKPGSITLEN ITRLCQTLGL ESFTEELSNE
      51  LSRLSTASKI IVIDVDYNKK QDRIQDVKLV LASNFDNFDY FNQRDGEHEK
     101  SNILLNSLTK YPDLKAFHNN LKFLYLLDAY SHIESDSTSH NNGSSDKSLD
     151  SSNASFNNQG KLDLFKYFTE LSHYIRQCFQ DNCCDFKVRT NLNDKFGIYI
     201  LTQGINGKEV PLAKIYLEEN KSDSQYRFYE YIYSQETKSW INESAENFSN
     251  GISLVMEIVA NAKESNYTDL IWFPEDFISP ELIIDKVTCS SNSSSSPPII
     301  DLFSNNNYNS RIQLMNDFTT KLINIKKFDI SNDNLDLISE ILKWVQWSRI
     351  VLQNVFKLVS TPSSNSNSSE LEPDYQAPFS TSTKDKNSST SNTEPIPRSN
     401  RHGSVVEASR RRRSSTNKSK RPSITEAMML KEEGLQQFNL HEILSEPAIE
     451  EENGDSIKEH STTMDGANDL GFTASVSNQE NAGTDIVMED HGVLQGTSQN
     501  YGTATADDAD IEMKDVSSKP SKPESSVLQL IVSEDHIILD TISECNLYDD
     551  VKCWSKFIEK FQDIVS*

external links for Med1p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	Organelle DB
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YPL+
YGOB	BioPIXIE	MIPS	YeastGFP
YOGY	CYC2008 (complexes)	Pfam domains	YeastRC Public Image Repository
	Complexome
	DIP
	GeneMANIA

References cited on this page View Complete Literature Guide for Med1p

1)	Balciunas D, et al. (1999) The Med1 subunit of the yeast mediator complex is involved in both transcriptional activation and repression. Proc Natl Acad Sci U S A 96(2):376-81
2)	Kornberg RD (2005) Mediator and the mechanism of transcriptional activation. Trends Biochem Sci 30(5):235-9
3)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41