UBA3/YPR066W Protein Information

Standard Name	Uba3p
Systematic Name	Ypr066wp
ORF Classification	Verified
Description	Protein that acts together with Ula1p to activate Rub1p before its conjugation to proteins (neddylation), which may play a role in protein degradation; GFP-fusion protein localizes to the cytoplasm in a punctate pattern (1, 2)
Name Description	UBiquitin-like protein-Activating enzyme 1

Experimental Data
Molecules/cell	752 3

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	299
Molecular Weight (Da)	33,278
Isoelectric Point (pI)	4.57

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Uba3p (InterPro)
Physical Interactions	There are 12 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI000006C2AE \| Q99344 MIPS: YPR066W NCBI: 1314134 \| 2980755 \| 46397007 \| 51013013 \| 6325323 \| 805046 \| NP_015391.1 GenBank/EMBL/DDBJ: DAA11486.1 \| AY692781 \| Y16891 \| Z49219 \| Z71255
External Classifications	EC: 6.3.2.- [Acid-Amino-Acid Ligases (Peptide Synthases)]

Amino Acid Sequence (or in FASTA format)
1 MDCKILVLGA GGLGCEILKN LTMLSFVKQV HIVDIDTIEL TNLNRQFLFC 51 DKDIGKPKAQ VAAQYVNTRF PQLEVVAHVQ DLTTLPPSFY KDFQFIISGL 101 DAIEPRRFIN ETLVKLTLES NYEICIPFID GGTEGLKGHV KTIIPGITAC 151 WECSIDTLPS QQDTVPMCTI ANNPRCIEHV VEYVSTIQYP DLNIESTADM 201 EFLLEKCCER AAQFSISTEK LSTSFILGII KSIIPSVSTT NAMVAATCCT 251 QMVKIYNDLI DLENGNNFTL INCSEGCFMY SFKFERLPDC TVCSNSNSN*

Amino Acid Sequence (or in FASTA format)

       1  MDCKILVLGA GGLGCEILKN LTMLSFVKQV HIVDIDTIEL TNLNRQFLFC
      51  DKDIGKPKAQ VAAQYVNTRF PQLEVVAHVQ DLTTLPPSFY KDFQFIISGL
     101  DAIEPRRFIN ETLVKLTLES NYEICIPFID GGTEGLKGHV KTIIPGITAC
     151  WECSIDTLPS QQDTVPMCTI ANNPRCIEHV VEYVSTIQYP DLNIESTADM
     201  EFLLEKCCER AAQFSISTEK LSTSFILGII KSIIPSVSTT NAMVAATCCT
     251  QMVKIYNDLI DLENGNNFTL INCSEGCFMY SFKFERLPDC TVCSNSNSN*

external links for Uba3p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
YGOB	BioPIXIE	MIPS	YeastRC Public Image Repository
YOGY	CYC2008 (complexes)	Pfam domains
	Complexome	YeastRC Structure Prediction (Seattle)
	DIP
	GeneMANIA

References cited on this page View Complete Literature Guide for Uba3p

1)	Liakopoulos D, et al. (1998) A novel protein modification pathway related to the ubiquitin system. EMBO J 17(8):2208-14
2)	Huh WK, et al. (2003) Global analysis of protein localization in budding yeast. Nature 425(6959):686-91
3)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41