SMK1/YPR054W Protein Information

Standard Name	Smk1p 1
Systematic Name	Ypr054wp
ORF Classification	Verified
Description	Middle sporulation-specific mitogen-activated protein kinase (MAPK) required for production of the outer spore wall layers; negatively regulates activity of the glucan synthase subunit Gsc2p (1, 2, 3, 4)

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	388
Molecular Weight (Da)	44,300
Isoelectric Point (pI)	8.87

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Smk1p (InterPro)
Physical Interactions	There are 23 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI0000135AAE \| P41808 MIPS: YPR054W NCBI: 1173459 \| 1314123 \| 538517 \| 6325311 \| 805035 \| NP_015379.1 GenBank/EMBL/DDBJ: DAA11476.1 \| L35047 \| Z49219 \| Z71255
External Classifications	EC: 2.7.11.24 [Mitogen-activated protein kinase]

Amino Acid Sequence (or in FASTA format)
1 MNCTLTDNTR AINVASNLGA PQQRTIFAKE RISIPGYYEI IQFLGKGAYG 51 TVCSVKFKGR SPAARIAVKK ISNIFNKEIL LKRAIRELKF MNFFKGHKNI 101 VNLIDLEIVT SSPYDGLYCY QELIDYDLAK VIHSSVQLSE FHIKYFLYQI 151 LCGLKYIHSA DVIHRDLKPG NILCTLNGCL KICDFGLARG IHAGFFKCHS 201 TVQPHITNYV ATRWYRAPEL LLSNQPYSKS VDIWAVGCIL AEFYARKPVF 251 MGRDSMHQIF EIIKVLGTPD KDILIKFGTI KAWNLGKNSN NPVYKKIPWS 301 NIFPFASHEA INLIESLLHW DSTHRLNVEQ AISHPFLNEV RKPDDEPVCL 351 QGPFDFTYES ELNSMSKLRD YLVEEVKNFK TDLSSSSL*

Amino Acid Sequence (or in FASTA format)

       1  MNCTLTDNTR AINVASNLGA PQQRTIFAKE RISIPGYYEI IQFLGKGAYG
      51  TVCSVKFKGR SPAARIAVKK ISNIFNKEIL LKRAIRELKF MNFFKGHKNI
     101  VNLIDLEIVT SSPYDGLYCY QELIDYDLAK VIHSSVQLSE FHIKYFLYQI
     151  LCGLKYIHSA DVIHRDLKPG NILCTLNGCL KICDFGLARG IHAGFFKCHS
     201  TVQPHITNYV ATRWYRAPEL LLSNQPYSKS VDIWAVGCIL AEFYARKPVF
     251  MGRDSMHQIF EIIKVLGTPD KDILIKFGTI KAWNLGKNSN NPVYKKIPWS
     301  NIFPFASHEA INLIESLLHW DSTHRLNVEQ AISHPFLNEV RKPDDEPVCL
     351  QGPFDFTYES ELNSMSKLRD YLVEEVKNFK TDLSSSSL*

external links for Smk1p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	Organelle DB
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YPL+
Candida (CGD)	BioPIXIE	MIPS	YeastGFP
Candida (CandidaDB)	CYC2008 (complexes)	Pfam domains
YGOB	Complexome	YeastRC Structure Prediction (Seattle)
YOGY	DIP
	GeneMANIA

References cited on this page View Complete Literature Guide for Smk1p

1)	Krisak L, et al. (1994) SMK1, a developmentally regulated MAP kinase, is required for spore wall assembly in Saccharomyces cerevisiae. Genes Dev 8(18):2151-61
2)	Wagner M, et al. (1999) Distinct steps in yeast spore morphogenesis require distinct SMK1 MAP kinase thresholds. Genetics 151(4):1327-40
3)	Pierce M, et al. (1998) Transcriptional regulation of the SMK1 mitogen-activated protein kinase gene during meiotic development in Saccharomyces cerevisiae. Mol Cell Biol 18(10):5970-80
4)	Huang LS, et al. (2005) The Smk1p MAP kinase negatively regulates Gsc2p, a 1,3-beta-glucan synthase, during spore wall morphogenesis in Saccharomyces cerevisiae. Proc Natl Acad Sci U S A 102(35):12431-6