MAK3/YPR051W Protein Information Help

Standard Name Mak3p 1
Systematic Name Ypr051wp
Alias Naa30p 2
ORF Classification Verified
Description Catalytic subunit of the NatC type N-terminal acetyltransferase; involved in subcellular targeting of select N-terminally acetylated substrates to the Golgi apparatus (Arl3p and Grh1p) and the inner nuclear membrane (Trm1p); required for replication of dsRNA virus (3, 4, 5, 6, 7)
Name Description MAintenance of Killer 8
Experimental Data
Molecules/cell 1940 9
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 176
Molecular Weight (Da) 20,457
Isoelectric Point (pI) 7.37

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Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Mak3p (InterPro)
Physical Interactions There are 17 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI000012EB33 | Q03503
MIPS: YPR051W
NCBI: 1314121 | 171882 | 417272 | 6325307 | 805033 | NP_015376.1 | NM_001184148.1
GenBank/EMBL/DDBJ: AAA34753.1 | CAA89170.1 | CAA94997.1 | DAA11474.1 | M95912 | Z49219 | Z71255
External Classifications EC: 2.3.1.88 [Peptide alpha-N-acetyltransferase]
Amino Acid Sequence (or in FASTA format)
       1  MEIVYKPLDI RNEEQFASIK KLIDADLSEP YSIYVYRYFL NQWPELTYIA
      51  VDNKSGTPNI PIGCIVCKMD PHRNVRLRGY IGMLAVESTY RGHGIAKKLV
     101  EIAIDKMQRE HCDEIMLETE VENSAALNLY EGMGFIRMKR MFRYYLNEGD
     151  AFKLILPLTE KSCTRSTFLM HGRLAT*                         

external links for Mak3p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily LoQate
Ashbya (AGD) BOND GPMdb (Mass Spec.) YPL+
Aspergillus (AspGD) CYC2008 (complexes) MIPS YeastGFP
Candida (CGD) Complexome Pfam domains YeastRC Public Image Repository
YGOB DIP YeastRC Structure Prediction (Seattle)
YOGY GeneMANIA


IMP


YeastRC Two-Hybrid (Seattle)

References cited on this page View Complete Literature Guide for Mak3p
1) Wickner RB and Toh-e A  (1982) [HOK], a new yeast non-Mendelian trait, enables a replication-defective killer plasmid to be maintained. Genetics 100(2):159-74
2) Polevoda B, et al.  (2009) A synopsis of eukaryotic Nalpha-terminal acetyltransferases: nomenclature, subunits and substrates. BMC Proc 3 Suppl 6:S2
3) Wickner RB and Leibowitz MJ  (1976) Chromosomal genes essential for replication of a double-stranded RNA plasmid of Saccharomyces cerevisiae: the killer character of yeast. J Mol Biol 105(3):427-43
4) Polevoda B and Sherman F  (2001) NatC Nalpha-terminal acetyltransferase of yeast contains three subunits, Mak3p, Mak10p, and Mak31p. J Biol Chem 276(23):20154-9
5) Behnia R, et al.  (2004) Targeting of the Arf-like GTPase Arl3p to the Golgi requires N-terminal acetylation and the membrane protein Sys1p. Nat Cell Biol 6(5):405-13
6) Setty SR, et al.  (2004) Golgi targeting of ARF-like GTPase Arl3p requires its Nalpha-acetylation and the integral membrane protein Sys1p. Nat Cell Biol 6(5):414-9
7) Behnia R, et al.  (2007) The yeast orthologue of GRASP65 forms a complex with a coiled-coil protein that contributes to ER to Golgi traffic. J Cell Biol 176(3):255-61
8) Wickner RB  (1974) Chromosomal and nonchromosomal mutations affecting the "killer character" of Saccharomyces cerevisiae. Genetics 76(3):423-32
9) Ghaemmaghami S, et al.  (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41