PUF2/YPR042C Protein Information Help

Standard Name Puf2p
Systematic Name Ypr042cp
ORF Classification Verified
Description mRNA-binding protein; member of the PUF protein family, which is defined by the presence of Pumilio homology domains that confer RNA binding activity; preferentially binds mRNAs encoding membrane-associated proteins; PUF2 has a paralog, JSN1, that arose from the whole genome duplication (1, 2)
Name Description PUmilio-homology domain Family 3
Experimental Data
Molecules/cell 377 4
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 1,075
Molecular Weight (Da) 119,507
Isoelectric Point (pI) 9.18

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Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Puf2p (InterPro)
Physical Interactions There are 20 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI0000035CED | Q12221
MIPS: YPR042C
NCBI: 1314114 | 1370299 | 3980208 | 6325299 | 74676554 | 805026 | NP_015367.1
GenBank/EMBL/DDBJ: DAA11467.1 | Z49219 | Z68111 | Z71255 | Z73616
Amino Acid Sequence (or in FASTA format)
 
       1  MDNKRLYNGN LSNIPEVIDP GITIPIYEED IRNDTRMNTN ARSVRVSDKR
      51  GRSSSTSPQK IGSYRTRAGR FSDTLTNLLP SISAKLHHSK KSTPVVVVPP
     101  TSSTPDSLNS TTYAPRVSSD SFTVATPLSL QSTTTRTRTR NNTVSSQITA
     151  SSSLTTDVGN ATSANIWSAN AESNTSSSPL FDYPLATSYF EPLTRFKSTD
     201  NYTLPQTAQL NSFLEKNGNP NIWSSAGNSN TDHLNTPIVN RQRSQSQSTT
     251  NRVYTDAPYY QQPAQNYQVQ VPPRVPKSTS ISPVILDDVD PASINWITAN
     301  QKVPLVNQIS ALLPTNTISI SNVFPLQPTQ QHQQNAVNLT STSLATLCSQ
     351  YGKVLSARTL RGLNMALVEF STVESAICAL EALQGKELSK VGAPSTVSFA
     401  RVLPMYEQPL NVNGFNNTPK QPLLQEQLNH GVLNYQLQQS LQQPELQQQP
     451  TSFNQPNLTY CNPTQNLSHL QLSSNENEPY PFPLPPPSLS DSKKDILHTI
     501  SSFKLEYDHL ELNHLLQNAL KNKGVSDTNY FGPLPEHNSK VPKRKDTFDA
     551  PKLRELRKQF DSNSLSTIEM EQLAIVMLDQ LPELSSDYLG NTVIQKLFEN
     601  SSNIIRDIML RKCNKYLTSM GVHKNGTWVC QKIIKMANTP RQINLVTSGV
     651  SDYCTPLFND QFGNYVIQGI LKFGFPWNSF IFESVLSHFW TIVQNRYGSR
     701  AVRACLEADS IITQCQLLTI TSLIIVLSPY LATDTNGTLL ITWLLDTCTL
     751  PNKNLILCDK LVNKNLVKLC CHKLGSLTVL KILNLRGGEE EALSKNKIIH
     801  AIFDGPISSD SILFQILDEG NYGPTFIYKV LTSRILDNSV RDEAITKIRQ
     851  LILNSNINLQ SRQLLEEVGL SSAGISPKQS SKNHRKQHPQ GFHSPGRARG
     901  VSVSSVRSSN SRHNSVIQMN NAGPTPALNF NPAPMSEINS YFNNQQVVYS
     951  GNQNQNQNGN SNGLDELNSQ FDSFRIANGT NLSLPIVNLP NVSNNNNNYN
    1001  NSGYSSQMNP LSRSVSHNNN NNTNNYNNND NDNNNNNNNN NNNNNNNNNN
    1051  NNNSNNSNNN NNNDTSLYRY RSYGY*

external links for Puf2p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily Organelle DB
Ashbya (AGD) BOND GPMdb (Mass Spec.) YPL+
YGOB BioPIXIE MIPS YeastGFP
YOGY CYC2008 (complexes) Pfam domains YeastRC Public Image Repository

Complexome YeastRC Structure Prediction (Seattle)

GeneMANIA

References cited on this page View Complete Literature Guide for Puf2p
1) Gerber AP, et al.  (2004) Extensive association of functionally and cytotopically related mRNAs with Puf family RNA-binding proteins in yeast. PLoS Biol 2(3):E79
2) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
3) Olivas W and Parker R  (2000) The Puf3 protein is a transcript-specific regulator of mRNA degradation in yeast. EMBO J 19(23):6602-11
4) Ghaemmaghami S, et al.  (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41