TIF5/YPR041W Protein Information Help

Standard Name Tif5p
Systematic Name Ypr041wp
Alias Sui5p 1
ORF Classification Verified
Description Translation initiation factor eIF5; functions both as a GTPase-activating protein to mediate hydrolysis of ribosome-bound GTP and as a GDP dissociation inhibitor to prevent recycling of eIF2 (2, 3, 4)
Name Description Translation Initiation Factor
Experimental Data
Molecules/cell 48300 5
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 405
Molecular Weight (Da) 45,261
Isoelectric Point (pI) 4.78

Click on image for expanded interactive view
pbrowse

Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Tif5p (InterPro)
Physical Interactions There are 177 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI0000053148 | P38431
MIPS: YPR041W
NCBI: 1072407 | 109157712 | 1314113 | 1370298 | 585304 | 6325298 | NP_015366.1 | NM_001184138.1
GenBank/EMBL/DDBJ: CAA92145.1 | CAA94989.1 | CAA97991.1 | DAA11466.1 | L10840 | Z68111 | Z71255 | Z73616
Amino Acid Sequence (or in FASTA format)
       1  MSINICRDNH DPFYRYKMPP IQAKVEGRGN GIKTAVLNVA DISHALNRPA
      51  PYIVKYFGFE LGAQTSISVD KDRYLVNGVH EPAKLQDVLD GFINKFVLCG
     101  SCKNPETEII ITKDNDLVRD CKACGKRTPM DLRHKLSSFI LKNPPDSVSG
     151  SKKKKKAATA SANVRGGGLS ISDIAQGKSQ NAPSDGTGSS TPQHHDEDED
     201  ELSRQIKAAA STLEDIEVKD DEWAVDMSEE AIRARAKELE VNSELTQLDE
     251  YGEWILEQAG EDKENLPSDV ELYKKAAELD VLNDPKIGCV LAQCLFDEDI
     301  VNEIAEHNAF FTKILVTPEY EKNFMGGIER FLGLEHKDLI PLLPKILVQL
     351  YNNDIISEEE IMRFGTKSSK KFVPKEVSKK VRRAAKPFIT WLETAESDDD
     401  EEDDE*                                                

external links for Tif5p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily LoQate
Ashbya (AGD) BOND GPMdb (Mass Spec.) YPL+
Aspergillus (AspGD) CYC2008 (complexes) MIPS YeastGFP
Candida (CGD) Complexome Pfam domains YeastRC Public Image Repository
YGOB DIP YeastRC Structure Prediction (Seattle)
YOGY GeneMANIA


IMP

References cited on this page View Complete Literature Guide for Tif5p
1) Huang HK, et al.  (1997) GTP hydrolysis controls stringent selection of the AUG start codon during translation initiation in Saccharomyces cerevisiae. Genes Dev 11(18):2396-413
2) Das S, et al.  (2001) Eukaryotic translation initiation factor 5 functions as a GTPase-activating protein. J Biol Chem 276(9):6720-6
3) Asano K, et al.  (2000) A multifactor complex of eukaryotic initiation factors, eIF1, eIF2, eIF3, eIF5, and initiator tRNA(Met) is an important translation initiation intermediate in vivo. Genes Dev 14(19):2534-46
4) Jennings MD and Pavitt GD  (2010) eIF5 has GDI activity necessary for translational control by eIF2 phosphorylation. Nature 465(7296):378-81
5) Ghaemmaghami S, et al.  (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41