TIF5/YPR041W Protein Information

Standard Name	Tif5p
Systematic Name	Ypr041wp
Alias	Sui5p 1
ORF Classification	Verified
Description	Translation initiation factor eIF5; functions both as a GTPase-activating protein to mediate hydrolysis of ribosome-bound GTP and as a GDP dissociation inhibitor to prevent recycling of eIF2 (2, 3, 4)
Name Description	Translation Initiation Factor

Experimental Data
Molecules/cell	48300 5

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	405
Molecular Weight (Da)	45,261
Isoelectric Point (pI)	4.78

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Tif5p (InterPro)
Physical Interactions	There are 173 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI0000053148 \| P38431 MIPS: YPR041W NCBI: 1072407 \| 109157712 \| 109157713 \| 109157714 \| 109157715 \| 109157716 \| 109157717 \| 1314113 \| 1370298 \| 585304 \| 6325298 \| NP_015366.1 GenBank/EMBL/DDBJ: DAA11466.1 \| L10840 \| Z68111 \| Z71255 \| Z73616

Amino Acid Sequence (or in FASTA format)
1 MSINICRDNH DPFYRYKMPP IQAKVEGRGN GIKTAVLNVA DISHALNRPA 51 PYIVKYFGFE LGAQTSISVD KDRYLVNGVH EPAKLQDVLD GFINKFVLCG 101 SCKNPETEII ITKDNDLVRD CKACGKRTPM DLRHKLSSFI LKNPPDSVSG 151 SKKKKKAATA SANVRGGGLS ISDIAQGKSQ NAPSDGTGSS TPQHHDEDED 201 ELSRQIKAAA STLEDIEVKD DEWAVDMSEE AIRARAKELE VNSELTQLDE 251 YGEWILEQAG EDKENLPSDV ELYKKAAELD VLNDPKIGCV LAQCLFDEDI 301 VNEIAEHNAF FTKILVTPEY EKNFMGGIER FLGLEHKDLI PLLPKILVQL 351 YNNDIISEEE IMRFGTKSSK KFVPKEVSKK VRRAAKPFIT WLETAESDDD 401 EEDDE*

Amino Acid Sequence (or in FASTA format)

       1  MSINICRDNH DPFYRYKMPP IQAKVEGRGN GIKTAVLNVA DISHALNRPA
      51  PYIVKYFGFE LGAQTSISVD KDRYLVNGVH EPAKLQDVLD GFINKFVLCG
     101  SCKNPETEII ITKDNDLVRD CKACGKRTPM DLRHKLSSFI LKNPPDSVSG
     151  SKKKKKAATA SANVRGGGLS ISDIAQGKSQ NAPSDGTGSS TPQHHDEDED
     201  ELSRQIKAAA STLEDIEVKD DEWAVDMSEE AIRARAKELE VNSELTQLDE
     251  YGEWILEQAG EDKENLPSDV ELYKKAAELD VLNDPKIGCV LAQCLFDEDI
     301  VNEIAEHNAF FTKILVTPEY EKNFMGGIER FLGLEHKDLI PLLPKILVQL
     351  YNNDIISEEE IMRFGTKSSK KFVPKEVSKK VRRAAKPFIT WLETAESDDD
     401  EEDDE*

external links for Tif5p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
Aspergillus (AspGD)	BioPIXIE	MIPS	YeastRC Public Image Repository
Candida (CGD)	CYC2008 (complexes)	Pfam domains
Candida (CandidaDB)	Complexome	YeastRC Structure Prediction (Seattle)
YGOB	DIP
YOGY	GeneMANIA

References cited on this page View Complete Literature Guide for Tif5p

1)	Huang HK, et al. (1997) GTP hydrolysis controls stringent selection of the AUG start codon during translation initiation in Saccharomyces cerevisiae. Genes Dev 11(18):2396-413
2)	Das S, et al. (2001) Eukaryotic translation initiation factor 5 functions as a GTPase-activating protein. J Biol Chem 276(9):6720-6
3)	Asano K, et al. (2000) A multifactor complex of eukaryotic initiation factors, eIF1, eIF2, eIF3, eIF5, and initiator tRNA(Met) is an important translation initiation intermediate in vivo. Genes Dev 14(19):2534-46
4)	Jennings MD and Pavitt GD (2010) eIF5 has GDI activity necessary for translational control by eIF2 phosphorylation. Nature 465(7296):378-81
5)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41