VMA13/YPR036W Protein Information

Standard Name	Vma13p
Systematic Name	Ypr036wp
Alias	Cls11p
ORF Classification	Verified
Description	Subunit H of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; serves as an activator or a structural stabilizer of the V-ATPase (1, 2, 3)

Experimental Data
Molecules/cell	22500 4

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	478
Molecular Weight (Da)	54,415
Isoelectric Point (pI)	6.31

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Vma13p (InterPro)
Physical Interactions	There are 87 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI000005306E \| P41807 MIPS: YPR036W NCBI: 1072404 \| 1174971 \| 1314110 \| 14719648 \| 439902 \| 6325293 \| NP_015361.1 GenBank/EMBL/DDBJ: DAA11462.1 \| D13916 \| Z68111 \| Z71255
External Classifications	EC: 3.6.3.14 [H(+)-transporting two-sector ATPase]

Amino Acid Sequence (or in FASTA format)
1 MGATKILMDS THFNEIRSII RSRSVAWDAL ARSEELSEID ASTAKALESI 51 LVKKNIGDGL SSSNNAHSGF KVNGKTLIPL IHLLSTSDNE DCKKSVQNLI 101 AELLSSDKYG DDTVKFFQED PKQLEQLFDV SLKGDFQTVL ISGFNVVSLL 151 VQNGLHNVKL VEKLLKNNNL INILQNIEQM DTCYVCIRLL QELAVIPEYR 201 DVIWLHEKKF MPTLFKILQR ATDSQLATRI VATNSNHLGI QLQYHSLLLI 251 WLLTFNPVFA NELVQKYLSD FLDLLKLVKI TIKEKVSRLC ISIILQCCST 301 RVKQHKKVIK QLLLLGNALP TVQSLSERKY SDEELRQDIS NLKEILENEY 351 QELTSFDEYV AELDSKLLCW SPPHVDNGFW SDNIDEFKKD NYKIFRQLIE 401 LLQAKVRNGD VNAKQEKIII QVALNDITHV VELLPESIDV LDKTGGKADI 451 MELLNHSDSR VKYEALKATQ AIIGYTFK*

Amino Acid Sequence (or in FASTA format)

       1  MGATKILMDS THFNEIRSII RSRSVAWDAL ARSEELSEID ASTAKALESI
      51  LVKKNIGDGL SSSNNAHSGF KVNGKTLIPL IHLLSTSDNE DCKKSVQNLI
     101  AELLSSDKYG DDTVKFFQED PKQLEQLFDV SLKGDFQTVL ISGFNVVSLL
     151  VQNGLHNVKL VEKLLKNNNL INILQNIEQM DTCYVCIRLL QELAVIPEYR
     201  DVIWLHEKKF MPTLFKILQR ATDSQLATRI VATNSNHLGI QLQYHSLLLI
     251  WLLTFNPVFA NELVQKYLSD FLDLLKLVKI TIKEKVSRLC ISIILQCCST
     301  RVKQHKKVIK QLLLLGNALP TVQSLSERKY SDEELRQDIS NLKEILENEY
     351  QELTSFDEYV AELDSKLLCW SPPHVDNGFW SDNIDEFKKD NYKIFRQLIE
     401  LLQAKVRNGD VNAKQEKIII QVALNDITHV VELLPESIDV LDKTGGKADI
     451  MELLNHSDSR VKYEALKATQ AIIGYTFK*

external links for Vma13p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	Organelle DB
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YPL+
Candida (CGD)	BioPIXIE	MIPS	YeastGFP
Candida (CandidaDB)	CYC2008 (complexes)	Pfam domains	YeastRC Public Image Repository
YGOB	Complexome	YeastRC Structure Prediction (Seattle)
YOGY	DIP
	GeneMANIA

References cited on this page View Complete Literature Guide for Vma13p

1)	Ho MN, et al. (1993) VMA13 encodes a 54-kDa vacuolar H(+)-ATPase subunit required for activity but not assembly of the enzyme complex in Saccharomyces cerevisiae. J Biol Chem 268(24):18286-92
2)	Forgac M (1999) Structure and properties of the vacuolar (H+)-ATPases. J Biol Chem 274(19):12951-4
3)	Keenan Curtis K and Kane PM (2002) Novel vacuolar H+-ATPase complexes resulting from overproduction of Vma5p and Vma13p. J Biol Chem 277(4):2716-24
4)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41