APL4/YPR029C Protein Information

Standard Name	Apl4p
Systematic Name	Ypr029cp
ORF Classification	Verified
Description	Gamma-adaptin, large subunit of the clathrin-associated protein (AP-1) complex; binds clathrin; involved in vesicle mediated transport (1, 2)
Name Description	clathrin Adaptor Protein complex Large chain

Experimental Data
Molecules/cell	4850 3

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	832
Molecular Weight (Da)	93,623
Isoelectric Point (pI)	5.17

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Apl4p (InterPro)
Physical Interactions	There are 63 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI000005327D \| Q12028 MIPS: YPR029C NCBI: 1314103 \| 6325286 \| 74583793 \| 809594 \| NP_015354.1 GenBank/EMBL/DDBJ: DAA11455.1 \| Z49274 \| Z71255

Amino Acid Sequence (or in FASTA format)
1 MGSSLRSFIK DVRGAKTLAD ERAIITKQSA KIRTKLRDDH LPHEKRRVNI 51 QKLLYLYILG EKTHFGQVES INLIASDDFV DKRLGYLAAT LLLDESEDLL 101 TLLTNMLNND LHHPNKYAVS LALTSLGFLS SPELARDLYP DVENIIKNSR 151 DPFLLKKALQ CAAKLIFKDV SLLEIFNIED ITKILSSHSI CTHGVLLGVT 201 KIIQSILLIG LNRKKDEDED EDGIDYSNDI LSPLSLLLRD FFIRLENMNS 251 KNIEPGYDVQ GICDPFLQCE IIYTLKLYFQ VGELLNSNNV LDYKDNFCDL 301 LTRIATNTDS TKNSGQAILY ETVKTIFSLD LNQPLRVLGI NILAKFLAGK 351 DNNTKYVSLN TLLKVVPQEP TAVQRHRKFI SHCLQDTDVS IRMRALELSF 401 AILDDSNLVE LVNELMKFLA KQDEDSKDLI IYTIDHLIDT FDTRVVKDES 451 WKLDVFFNIL KLVGSFINYE KINDILIIIN NTSQLSDKSE FLRKMLTISL 501 NGTSAEISEE NIGWQLVLIW CIGEYGDLVL NEGNKNGADI INESSITDYL 551 LTLQELYTAT NLKIINYILT AALKLSVRFH DAKNIEKLRQ LILSYTDSTD 601 LSLQMKSNQY EIFFNQSISV KKIILETMPK FEKITEEQDN GKALSKNLIS 651 NEPVDLLSDL LGEDSKAESK ASTGDNVKPI DILEEIFGEK NDIAQVPKNA 701 NKEESINHSS AVEANSGVTL PLDANKIYDS SSLNVYASLL SANSGLAHLD 751 LYFQAKSLIS DLKTFCAVPK AQKLTLGQLY PSSTINASQI CKQSLKISGS 801 GKLKLRVKLD FHLNGSSSIT NEQFDHKFDE TL*

Amino Acid Sequence (or in FASTA format)

       1  MGSSLRSFIK DVRGAKTLAD ERAIITKQSA KIRTKLRDDH LPHEKRRVNI
      51  QKLLYLYILG EKTHFGQVES INLIASDDFV DKRLGYLAAT LLLDESEDLL
     101  TLLTNMLNND LHHPNKYAVS LALTSLGFLS SPELARDLYP DVENIIKNSR
     151  DPFLLKKALQ CAAKLIFKDV SLLEIFNIED ITKILSSHSI CTHGVLLGVT
     201  KIIQSILLIG LNRKKDEDED EDGIDYSNDI LSPLSLLLRD FFIRLENMNS
     251  KNIEPGYDVQ GICDPFLQCE IIYTLKLYFQ VGELLNSNNV LDYKDNFCDL
     301  LTRIATNTDS TKNSGQAILY ETVKTIFSLD LNQPLRVLGI NILAKFLAGK
     351  DNNTKYVSLN TLLKVVPQEP TAVQRHRKFI SHCLQDTDVS IRMRALELSF
     401  AILDDSNLVE LVNELMKFLA KQDEDSKDLI IYTIDHLIDT FDTRVVKDES
     451  WKLDVFFNIL KLVGSFINYE KINDILIIIN NTSQLSDKSE FLRKMLTISL
     501  NGTSAEISEE NIGWQLVLIW CIGEYGDLVL NEGNKNGADI INESSITDYL
     551  LTLQELYTAT NLKIINYILT AALKLSVRFH DAKNIEKLRQ LILSYTDSTD
     601  LSLQMKSNQY EIFFNQSISV KKIILETMPK FEKITEEQDN GKALSKNLIS
     651  NEPVDLLSDL LGEDSKAESK ASTGDNVKPI DILEEIFGEK NDIAQVPKNA
     701  NKEESINHSS AVEANSGVTL PLDANKIYDS SSLNVYASLL SANSGLAHLD
     751  LYFQAKSLIS DLKTFCAVPK AQKLTLGQLY PSSTINASQI CKQSLKISGS
     801  GKLKLRVKLD FHLNGSSSIT NEQFDHKFDE TL*

external links for Apl4p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	Organelle DB
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YPL+
YGOB	BioPIXIE	MIPS	YeastGFP
YOGY	CYC2008 (complexes)	Pfam domains	YeastRC Public Image Repository
	Complexome	YeastRC Structure Prediction (Seattle)
	DIP
	GeneMANIA

References cited on this page View Complete Literature Guide for Apl4p

1)	Panek HR, et al. (1997) Suppressors of YCK-encoded yeast casein kinase 1 deficiency define the four subunits of a novel clathrin AP-like complex. EMBO J 16(14):4194-204
2)	Kirchhausen T (2000) Three ways to make a vesicle. Nat Rev Mol Cell Biol 1(3):187-98
3)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41