MCM4/YPR019W Protein Information Help

Standard Name Mcm4p 1
Systematic Name Ypr019wp
Alias Hcd21p 2 , Cdc54p 3
ORF Classification Verified
Description Essential helicase component of heterohexameric MCM2-7 complexes which bind pre-replication complexes on DNA and melt DNA prior to replication; forms an Mcm4p-6p-7p subcomplex; shows nuclear accumulation in G1; homolog of S. pombe Cdc21p (4, 5, 6)
Name Description MiniChromosome Maintenance 1
Experimental Data
Molecules/cell 8800 7
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 933
Molecular Weight (Da) 105,002
Isoelectric Point (pI) 6.13

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Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Mcm4p (InterPro)
Physical Interactions There are 172 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI0000127256 | P30665
MIPS: YPR019W
NCBI: 1168816 | 1314093 | 3482 | 608171 | 6325276 | 887597 | NP_015344.1
GenBank/EMBL/DDBJ: DAA11445.1 | U14731 | Z15032 | Z49919 | Z71255
Amino Acid Sequence (or in FASTA format)
 
       1  MSQQSSSPTK EDNNSSSPVV PNPDSVPPQL SSPALFYSSS SSQGDIYGRN
      51  NSQNLSQGEG NIRAAIGSSP LNFPSSSQRQ NSDVFQSQGR QGRIRSSASA
     101  SGRSRYHSDL RSDRALPTSS SSLGRNGQNR VHMRRNDIHT SDLSSPRRIV
     151  DFDTRSGVNT LDTSSSSAPP SEASEPLRII WGTNVSIQEC TTNFRNFLMS
     201  FKYKFRKILD EREEFINNTT DEELYYIKQL NEMRELGTSN LNLDARNLLA
     251  YKQTEDLYHQ LLNYPQEVIS IMDQTIKDCM VSLIVDNNLD YDLDEIETKF
     301  YKVRPYNVGS CKGMRELNPN DIDKLINLKG LVLRSTPVIP DMKVAFFKCN
     351  VCDHTMAVEI DRGVIQEPAR CERIDCNEPN SMSLIHNRCS FADKQVIKLQ
     401  ETPDFVPDGQ TPHSISLCVY DELVDSCRAG DRIEVTGTFR SIPIRANSRQ
     451  RVLKSLYKTY VDVVHVKKVS DKRLDVDTST IEQELMQNKV DHNEVEEVRQ
     501  ITDQDLAKIR EVAAREDLYS LLARSIAPSI YELEDVKKGI LLQLFGGTNK
     551  TFTKGGRYRG DINILLCGDP STSKSQILQY VHKITPRGVY TSGKGSSAVG
     601  LTAYITRDVD TKQLVLESGA LVLSDGGVCC IDEFDKMSDS TRSVLHEVME
     651  QQTISIAKAG IITTLNARSS ILASANPIGS RYNPNLPVTE NIDLPPPLLS
     701  RFDLVYLVLD KVDEKNDREL AKHLTNLYLE DKPEHISQDD VLPVEFLTMY
     751  ISYAKEHIHP IITEAAKTEL VRAYVGMRKM GDDSRSDEKR ITATTRQLES
     801  MIRLAEAHAK MKLKNVVELE DVQEAVRLIR SAIKDYATDP KTGKIDMNLV
     851  QTGKSVIQRK LQEDLSREIM NVLKDQASDS MSFNELIKQI NEHSQDRVES
     901  SDIQEALSRL QQEDKVIVLG EGVRRSVRLN NRV*

external links for Mcm4p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily YPL+
Ashbya (AGD) BOND GPMdb (Mass Spec.) YeastGFP
Aspergillus (AspGD) BioPIXIE MIPS YeastRC Public Image Repository
Candida (CGD) CYC2008 (complexes) Pfam domains
Candida (CandidaDB) Complexome YeastRC Structure Prediction (Seattle)
YGOB DIP

YOGY GeneMANIA

References cited on this page View Complete Literature Guide for Mcm4p
1) Merchant AM, et al.  (1997) A lesion in the DNA replication initiation factor Mcm10 induces pausing of elongation forks through chromosomal replication origins in Saccharomyces cerevisiae. Mol Cell Biol 17(6):3261-71
2) Coxon A, et al.  (1992) Fission yeast cdc21+ belongs to a family of proteins involved in an early step of chromosome replication. Nucleic Acids Res 20(21):5571-7
3) Hennessy KM, et al.  (1991) A group of interacting yeast DNA replication genes. Genes Dev 5(6):958-69
4) Tye BK  (1999) MCM proteins in DNA replication. Annu Rev Biochem 68:649-86
5) Tanaka S and Diffley JF  (2002) Interdependent nuclear accumulation of budding yeast Cdt1 and Mcm2-7 during G1 phase. Nat Cell Biol 4(3):198-207
6) Ma X, et al.  (2010) The effects of oligomerization on Saccharomyces cerevisiae Mcm4/6/7 function. BMC Biochem 11():37
7) Ghaemmaghami S, et al.  (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41