RPA135/YPR010C Protein Information

Standard Name	Rpa135p 1
Systematic Name	Ypr010cp
Alias	Rpa2p 2 , Rrn2p 3 , Srp3p 1, 4
ORF Classification	Verified
Description	RNA polymerase I second largest subunit A135 (1, 3)
Name Description	RNA Polymerase A 1
Gene Product	A135 5

Experimental Data
Molecules/cell	14100 6

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	1,203
Molecular Weight (Da)	135,741
Isoelectric Point (pI)	7.61

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Rpa135p (InterPro)
Physical Interactions	There are 228 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI0000052E84 \| P22138 MIPS: YPR010C NCBI: 1314085 \| 133320 \| 172464 \| 6325267 \| 887587 \| 939744 \| NP_015335.1 \| NM_001184107.1 GenBank/EMBL/DDBJ: DAA11437.1 \| M62804 \| U31900 \| Z49919 \| Z71255
External Classifications	EC: 2.7.7.6 [DNA-directed RNA polymerase]

Amino Acid Sequence (or in FASTA format)
1 MSKVIKPPGQ ARTADFRTLE RESRFINPPK DKSAFPLLQE AVQPHIGSFN 51 ALTEGPDGGL LNLGVKDIGE KVIFDGKPLN SEDEISNSGY LGNKLSVSVE 101 QVSIAKPMSN DGVSSAVERK VYPSESRQRL TSYRGKLLLK LKWSVNNGEE 151 NLFEVRDCGG LPVMLQSNRC HLNKMSPYEL VQHKEESDEI GGYFIVNGIE 201 KLIRMLIVQR RNHPMAIIRP SFANRGASYS HYGIQIRSVR PDQTSQTNVL 251 HYLNDGQVTF RFSWRKNEYL VPVVMILKAL CHTSDREIFD GIIGNDVKDS 301 FLTDRLELLL RGFKKRYPHL QNRTQVLQYL GDKFRVVFQA SPDQSDLEVG 351 QEVLDRIVLV HLGKDGSQDK FRMLLFMIRK LYSLVAGECS PDNPDATQHQ 401 EVLLGGFLYG MILKEKIDEY LQNIIAQVRM DINRGMAINF KDKRYMSRVL 451 MRVNENIGSK MQYFLSTGNL VSQSGLDLQQ VSGYTVVAEK INFYRFISHF 501 RMVHRGSFFA QLKTTTVRKL LPESWGFLCP VHTPDGSPCG LLNHFAHKCR 551 ISTQQSDVSR IPSILYSLGV APASHTFAAG PSLCCVQIDG KIIGWVSHEQ 601 GKIIADTLRY WKVEGKTPGL PIDLEIGYVP PSTRGQYPGL YLFGGHSRML 651 RPVRYLPLDK EDIVGPFEQV YMNIAVTPQE IQNNVHTHVE FTPTNILSIL 701 ANLTPFSDFN QSPRNMYQCQ MGKQTMGTPG VALCHRSDNK LYRLQTGQTP 751 IVKANLYDDY GMDNFPNGFN AVVAVISYTG YDMDDAMIIN KSADERGFGY 801 GTMYKTEKVD LALNRNRGDP ITQHFGFGND EWPKEWLEKL DEDGLPYIGT 851 YVEEGDPICA YFDDTLNKTK IKTYHSSEPA YIEEVNLIGD ESNKFQELQT 901 VSIKYRIRRT PQIGDKFSSR HGQKGVCSRK WPTIDMPFSE TGIQPDIIIN 951 PHAFPSRMTI GMFVESLAGK AGALHGIAQD STPWIFNEDD TPADYFGEQL 1001 AKAGYNYHGN EPMYSGATGE ELRADIYVGV VYYQRLRHMV NDKFQVRSTG 1051 PVNSLTMQPV KGRKRHGGIR VGEMERDALI GHGTSFLLQD RLLNSSDYTQ 1101 ASVCRECGSI LTTQQSVPRI GSISTVCCRR CSMRFEDAKK LLTKSEDGEK 1151 IFIDDSQIWE DGQGNKFVGG NETTTVAIPF VLKYLDSELS AMGIRLRYNV 1201 EPK*

Amino Acid Sequence (or in FASTA format)

       1  MSKVIKPPGQ ARTADFRTLE RESRFINPPK DKSAFPLLQE AVQPHIGSFN
      51  ALTEGPDGGL LNLGVKDIGE KVIFDGKPLN SEDEISNSGY LGNKLSVSVE
     101  QVSIAKPMSN DGVSSAVERK VYPSESRQRL TSYRGKLLLK LKWSVNNGEE
     151  NLFEVRDCGG LPVMLQSNRC HLNKMSPYEL VQHKEESDEI GGYFIVNGIE
     201  KLIRMLIVQR RNHPMAIIRP SFANRGASYS HYGIQIRSVR PDQTSQTNVL
     251  HYLNDGQVTF RFSWRKNEYL VPVVMILKAL CHTSDREIFD GIIGNDVKDS
     301  FLTDRLELLL RGFKKRYPHL QNRTQVLQYL GDKFRVVFQA SPDQSDLEVG
     351  QEVLDRIVLV HLGKDGSQDK FRMLLFMIRK LYSLVAGECS PDNPDATQHQ
     401  EVLLGGFLYG MILKEKIDEY LQNIIAQVRM DINRGMAINF KDKRYMSRVL
     451  MRVNENIGSK MQYFLSTGNL VSQSGLDLQQ VSGYTVVAEK INFYRFISHF
     501  RMVHRGSFFA QLKTTTVRKL LPESWGFLCP VHTPDGSPCG LLNHFAHKCR
     551  ISTQQSDVSR IPSILYSLGV APASHTFAAG PSLCCVQIDG KIIGWVSHEQ
     601  GKIIADTLRY WKVEGKTPGL PIDLEIGYVP PSTRGQYPGL YLFGGHSRML
     651  RPVRYLPLDK EDIVGPFEQV YMNIAVTPQE IQNNVHTHVE FTPTNILSIL
     701  ANLTPFSDFN QSPRNMYQCQ MGKQTMGTPG VALCHRSDNK LYRLQTGQTP
     751  IVKANLYDDY GMDNFPNGFN AVVAVISYTG YDMDDAMIIN KSADERGFGY
     801  GTMYKTEKVD LALNRNRGDP ITQHFGFGND EWPKEWLEKL DEDGLPYIGT
     851  YVEEGDPICA YFDDTLNKTK IKTYHSSEPA YIEEVNLIGD ESNKFQELQT
     901  VSIKYRIRRT PQIGDKFSSR HGQKGVCSRK WPTIDMPFSE TGIQPDIIIN
     951  PHAFPSRMTI GMFVESLAGK AGALHGIAQD STPWIFNEDD TPADYFGEQL
    1001  AKAGYNYHGN EPMYSGATGE ELRADIYVGV VYYQRLRHMV NDKFQVRSTG
    1051  PVNSLTMQPV KGRKRHGGIR VGEMERDALI GHGTSFLLQD RLLNSSDYTQ
    1101  ASVCRECGSI LTTQQSVPRI GSISTVCCRR CSMRFEDAKK LLTKSEDGEK
    1151  IFIDDSQIWE DGQGNKFVGG NETTTVAIPF VLKYLDSELS AMGIRLRYNV
    1201  EPK*

external links for Rpa135p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
Candida (CGD)	BioPIXIE	MIPS	YeastRC Public Image Repository
Candida (CandidaDB)	CYC2008 (complexes)	Pfam domains
YGOB	Complexome	YeastRC Structure Prediction (Seattle)
YOGY	DIP
	GeneMANIA

References cited on this page View Complete Literature Guide for Rpa135p

1)	Yano R and Nomura M (1991) Suppressor analysis of temperature-sensitive mutations of the largest subunit of RNA polymerase I in Saccharomyces cerevisiae: a suppressor gene encodes the second-largest subunit of RNA polymerase I. Mol Cell Biol 11(2):754-64
2)	Nonet M, et al. (1987) Eucaryotic RNA polymerase conditional mutant that rapidly ceases mRNA synthesis. Mol Cell Biol 7(5):1602-11
3)	Nogi Y, et al. (1991) An approach for isolation of mutants defective in 35S ribosomal RNA synthesis in Saccharomyces cerevisiae. Proc Natl Acad Sci U S A 88(16):7026-30
4)	McCusker JH, et al. (1991) Suppressor analysis of temperature-sensitive RNA polymerase I mutations in Saccharomyces cerevisiae: suppression of mutations in a zinc-binding motif by transposed mutant genes. Mol Cell Biol 11(2):746-53
5)	Riva M, et al. (1982) Natural variation in yeast RNA polymerase A. Formation of a mosaic RNA polymerase A in a meiotic segregant from an interspecific hybrid. J Biol Chem 257(8):4570-7
6)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41