YRF1-7/YPL283C Protein Information

Standard Name	Yrf1-7p
Systematic Name	Ypl283cp
Alias	Yrf1p
ORF Classification	Verified
Description	Helicase encoded by the Y' element of subtelomeric regions, highly expressed in the mutants lacking the telomerase component TLC1; potentially phosphorylated by Cdc28p (1, 2)

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	1,859
Molecular Weight (Da)	211,113
Isoelectric Point (pI)	7.19

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Yrf1-7p (InterPro)
Signal Peptide(s)	There are 1 total predicted predicted signal peptide(s) (SignalP)
Physical Interactions	There are 4 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI000013BFCF \| P0CX15 MIPS: YPL283C NCBI: 1370582 \| 2326849 \| 338819803 \| 398366437 \| 6324972 \| NP_011812.3 \| NP_015040.1 \| NM_001181425.3 \| NM_001184097.1 GenBank/EMBL/DDBJ: DAA11155.1 \| Z73521 \| Z73638
External Classifications	EC: 3.6.1.- [Hydrolases acting on acid anhydrides in phosphorous-containing anhydrides] EC: 3.6.4.12 [DNA helicase]

Amino Acid Sequence (or in FASTA format)
1 MEIENEQICT CIAQILHLLN SLIITFLDDD KTETGQSFVY IDGFLVKKHN 51 NQHTIVNFET YKNKMKVSDR RKFEKANFDE FESALNNKND LVHCPSITLF 101 ESIPTEVRSF YEDEKSGLIK VVKFRTGAMD RKRSFEKIVV SVMVGKNVQK 151 FLTFVEDEPD FQGGPIPSKY LIPKKINLMV YTLFQVHTLK FNRKDYDTLS 201 LFYLNRGYYN ELSFRVLERC YEIASARPND SSTMRTFTDF VSGTPIVRGL 251 QKSTIRKYGY NLAPYMFLLL HVDELSIFSA YQASLPGEKK VDTERLKRDL 301 CPRKPTEIKY FSQICNDMMN KKDRLGDILH IILRACALNF GAGPRGGAGD 351 EEDRSITNEE PIIPSVDEHG LKVCKLRSPN TPRRLRKTLD AVKALLVSSC 401 ACTARDLDIF DDNNGVAMWK WIKILYHEVA QETALKDSYR ITLVPSSDGV 451 SVCGKLFNRE YVRGFYFACK AQFDNLWEEL NDCFYMPTVV DIASLILRNR 501 EVLFREPKRG IDEYLENDSF LQMIPVKYRE IVLPKLRRDT NKMTAALKNK 551 VTVAIDELTV PLMWMIHFAV GYPYRYPELQ LLAFAGPQRN VYVDDTTRRI 601 QLYTDYNKNG SSEPRLKTLD GLTSDYVFYF VTVLRQMQIC ALGNSYDAFN 651 HDPWMDVVGF EDPDQVTNRD ISRIVLYSYM FLNTAKGCLV EYATFRQYMR 701 ELPKNAPQKL NFREMRQGLI ALGRHCVGSR FETDLYESAT SELMANHSVQ 751 TGRNIYGVDS FSLTSVSGTT ATLLQERASE RWIQWLGLES DYHCSFSSTR 801 NAEDVVAGEA ASSDHHQKIS RVTRKRPREP KSTNDILVAG QKLFGSSFEF 851 RDLHQLRLCH EIYMADTPSV AVQAPPGYGK TELFHLPLIA LASKGDVKYV 901 SFLFVPYTVL LANCMIRLSR CGCLNVAPVR NFIEEGCDGV TDLYVGIYDD 951 LASTNFTDRI AAWENIVECT FRTNNVKLGY LIVDEFHNFE TEVYRQSQFG 1001 GITNLDFDAF EKAIFLSGTA PEAVADAALQ RIGLTGLAKK SMDINELKRS 1051 EDLSRGLSSY PTRMFNLIKE KSEVPLGHVH KIWKKVESQP EEALKLLLAL 1101 FEIEPESKAI VVASTTNEVE ELACSWRKYF RVVWIHGKLG AAEKVSRTKE 1151 FVTDGSMRVL IGTKLVTEGI DIKQLMMVIM LDNRLNIIEL IQGVGRLRDG 1201 GLCYLLSRKN SWAARNRKGE LPPIKEGCIT EQVREFYGLE SKKGKKGQHV 1251 GCCGSRTDLS ADTVELIERM DRLAEKQATA SMSIVALPSS FQESNSSDRC 1301 RKYCSSDEDS DTCIHGSANA STNATTNSST NATTTASTNV RTSATTTASI 1351 NVRTSATTTE STNSSTNATT TASTNVRTSA TTTASINVRT SATTTESTNS 1401 NTSATTTEST DSNTSATTTE STDSNTSATT TASTNSSTNA TTTASTNSST 1451 NATTTESTNA SAKEDANKDG NAEDNRFHPV TDINKESYKR KGSQMVLLER 1501 KKLKAQFPNT SENMNVLQFL GFRSDEIKHL FLYGIDVYFC PEGVFTQYGL 1551 CKGCQKMFEL CVCWAGQKVS YRRMAWEALA VERMLRNDEE YKEYLEDIEP 1601 YHGDPVGYLK YFSVKRGEIY SQIQRNYAWY LAITRRRETI SVLDSTRGKQ 1651 GSQVFRMSGR QIKELYYKVW SNLRESKTEV LQYFLNWDEK KCREEWEAKD 1701 DTVFVEALEK VGVFQRLRSM TSAGLQGPQY VKLQFSRHHR QLRSRYELSL 1751 GMHLRDQLAL GVTPSKVPHW TAFLSMLIGL FCNKTFRQKL EYLLEQISEV 1801 WLLPHWLDLA NVEVLAADNT RVPLYMLMVA VHKELDSDDV PDGRFDILLC 1851 RDSSREVGE*

Amino Acid Sequence (or in FASTA format)

       1  MEIENEQICT CIAQILHLLN SLIITFLDDD KTETGQSFVY IDGFLVKKHN
      51  NQHTIVNFET YKNKMKVSDR RKFEKANFDE FESALNNKND LVHCPSITLF
     101  ESIPTEVRSF YEDEKSGLIK VVKFRTGAMD RKRSFEKIVV SVMVGKNVQK
     151  FLTFVEDEPD FQGGPIPSKY LIPKKINLMV YTLFQVHTLK FNRKDYDTLS
     201  LFYLNRGYYN ELSFRVLERC YEIASARPND SSTMRTFTDF VSGTPIVRGL
     251  QKSTIRKYGY NLAPYMFLLL HVDELSIFSA YQASLPGEKK VDTERLKRDL
     301  CPRKPTEIKY FSQICNDMMN KKDRLGDILH IILRACALNF GAGPRGGAGD
     351  EEDRSITNEE PIIPSVDEHG LKVCKLRSPN TPRRLRKTLD AVKALLVSSC
     401  ACTARDLDIF DDNNGVAMWK WIKILYHEVA QETALKDSYR ITLVPSSDGV
     451  SVCGKLFNRE YVRGFYFACK AQFDNLWEEL NDCFYMPTVV DIASLILRNR
     501  EVLFREPKRG IDEYLENDSF LQMIPVKYRE IVLPKLRRDT NKMTAALKNK
     551  VTVAIDELTV PLMWMIHFAV GYPYRYPELQ LLAFAGPQRN VYVDDTTRRI
     601  QLYTDYNKNG SSEPRLKTLD GLTSDYVFYF VTVLRQMQIC ALGNSYDAFN
     651  HDPWMDVVGF EDPDQVTNRD ISRIVLYSYM FLNTAKGCLV EYATFRQYMR
     701  ELPKNAPQKL NFREMRQGLI ALGRHCVGSR FETDLYESAT SELMANHSVQ
     751  TGRNIYGVDS FSLTSVSGTT ATLLQERASE RWIQWLGLES DYHCSFSSTR
     801  NAEDVVAGEA ASSDHHQKIS RVTRKRPREP KSTNDILVAG QKLFGSSFEF
     851  RDLHQLRLCH EIYMADTPSV AVQAPPGYGK TELFHLPLIA LASKGDVKYV
     901  SFLFVPYTVL LANCMIRLSR CGCLNVAPVR NFIEEGCDGV TDLYVGIYDD
     951  LASTNFTDRI AAWENIVECT FRTNNVKLGY LIVDEFHNFE TEVYRQSQFG
    1001  GITNLDFDAF EKAIFLSGTA PEAVADAALQ RIGLTGLAKK SMDINELKRS
    1051  EDLSRGLSSY PTRMFNLIKE KSEVPLGHVH KIWKKVESQP EEALKLLLAL
    1101  FEIEPESKAI VVASTTNEVE ELACSWRKYF RVVWIHGKLG AAEKVSRTKE
    1151  FVTDGSMRVL IGTKLVTEGI DIKQLMMVIM LDNRLNIIEL IQGVGRLRDG
    1201  GLCYLLSRKN SWAARNRKGE LPPIKEGCIT EQVREFYGLE SKKGKKGQHV
    1251  GCCGSRTDLS ADTVELIERM DRLAEKQATA SMSIVALPSS FQESNSSDRC
    1301  RKYCSSDEDS DTCIHGSANA STNATTNSST NATTTASTNV RTSATTTASI
    1351  NVRTSATTTE STNSSTNATT TASTNVRTSA TTTASINVRT SATTTESTNS
    1401  NTSATTTEST DSNTSATTTE STDSNTSATT TASTNSSTNA TTTASTNSST
    1451  NATTTESTNA SAKEDANKDG NAEDNRFHPV TDINKESYKR KGSQMVLLER
    1501  KKLKAQFPNT SENMNVLQFL GFRSDEIKHL FLYGIDVYFC PEGVFTQYGL
    1551  CKGCQKMFEL CVCWAGQKVS YRRMAWEALA VERMLRNDEE YKEYLEDIEP
    1601  YHGDPVGYLK YFSVKRGEIY SQIQRNYAWY LAITRRRETI SVLDSTRGKQ
    1651  GSQVFRMSGR QIKELYYKVW SNLRESKTEV LQYFLNWDEK KCREEWEAKD
    1701  DTVFVEALEK VGVFQRLRSM TSAGLQGPQY VKLQFSRHHR QLRSRYELSL
    1751  GMHLRDQLAL GVTPSKVPHW TAFLSMLIGL FCNKTFRQKL EYLLEQISEV
    1801  WLLPHWLDLA NVEVLAADNT RVPLYMLMVA VHKELDSDDV PDGRFDILLC
    1851  RDSSREVGE*

external links for Yrf1-7p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
YGOB	BioPIXIE	MIPS
YOGY	CYC2008 (complexes)	Pfam domains
	Complexome	YeastRC Structure Prediction (Seattle)
	GeneMANIA

References cited on this page View Complete Literature Guide for Yrf1-7p

1)	Yamada M, et al. (1998) Y'-Help1, a DNA helicase encoded by the yeast subtelomeric Y' element, is induced in survivors defective for telomerase. J Biol Chem 273(50):33360-6
2)	Ubersax JA, et al. (2003) Targets of the cyclin-dependent kinase Cdk1. Nature 425(6960):859-64