APM1/YPL259C Protein Information

Standard Name	Apm1p 1
Systematic Name	Ypl259cp
Alias	Yap54p 1
ORF Classification	Verified
Description	Mu1-like medium subunit of the clathrin-associated protein complex (AP-1); binds clathrin; involved in clathrin-dependent Golgi protein sorting (1, 2)
Name Description	clathrin Adaptor Protein complex Medium chain 1

Experimental Data
Molecules/cell	4420 3

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	475
Molecular Weight (Da)	53,873
Isoelectric Point (pI)	9.05

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Apm1p (InterPro)
Physical Interactions	There are 55 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI0000052FB3 \| Q00776 MIPS: YPL259C NCBI: 1370536 \| 1703330 \| 4800 \| 6324996 \| NP_015064.1 GenBank/EMBL/DDBJ: DAA11176.1 \| X60288 \| Z73615

Amino Acid Sequence (or in FASTA format)
1 MASAVYFCDH NGKPLLSRRY RDDIPLSAID KFPILLSDLE EQSNLIPPCL 51 NHNGLEYLFI QHNDLYVVAI VTSLSANAAA IFTFLHKLVE VLSDYLKTVE 101 EESIRDNFVI IYELLDEVMD YGIPQITETK MLKQYITQKS FKLVKSAKKK 151 RNATRPPVAL TNSVSWRPEG ITHKKNEAFL DIVESINMLM TQKGQVLRSE 201 IIGDVKVNSK LSGMPDLKLG INDKGIFSKY LDDDTNIPSA SATTSDNNTE 251 TDKKPSITSS SATNKKKVNI ELEDLKFHQC VRLSKFENEK IITFIPPDGK 301 FDLMNYRLST TIKPLIWCDV NVQVHSNSRI EIHCKAKAQI KRKSTATNVE 351 ILIPVPDDAD TPTFKYSHGS LKYVPEKSAI LWKIRSFPGG KEYSMSAELG 401 LPSISNNEDG NRTMPKSNAE ILKGPVQIKF QIPYFTTSGI QVRYLKINEP 451 KLQYKSYPWV RYITQSGDDY TIRLT*

Amino Acid Sequence (or in FASTA format)

       1  MASAVYFCDH NGKPLLSRRY RDDIPLSAID KFPILLSDLE EQSNLIPPCL
      51  NHNGLEYLFI QHNDLYVVAI VTSLSANAAA IFTFLHKLVE VLSDYLKTVE
     101  EESIRDNFVI IYELLDEVMD YGIPQITETK MLKQYITQKS FKLVKSAKKK
     151  RNATRPPVAL TNSVSWRPEG ITHKKNEAFL DIVESINMLM TQKGQVLRSE
     201  IIGDVKVNSK LSGMPDLKLG INDKGIFSKY LDDDTNIPSA SATTSDNNTE
     251  TDKKPSITSS SATNKKKVNI ELEDLKFHQC VRLSKFENEK IITFIPPDGK
     301  FDLMNYRLST TIKPLIWCDV NVQVHSNSRI EIHCKAKAQI KRKSTATNVE
     351  ILIPVPDDAD TPTFKYSHGS LKYVPEKSAI LWKIRSFPGG KEYSMSAELG
     401  LPSISNNEDG NRTMPKSNAE ILKGPVQIKF QIPYFTTSGI QVRYLKINEP
     451  KLQYKSYPWV RYITQSGDDY TIRLT*

external links for Apm1p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
Candida (CGD)	BioPIXIE	MIPS	YeastRC Public Image Repository
Candida (CandidaDB)	CYC2008 (complexes)	Pfam domains
YGOB	Complexome	YeastRC Structure Prediction (Seattle)
YOGY	DIP
	GeneMANIA

References cited on this page View Complete Literature Guide for Apm1p

1)	Nakayama Y, et al. (1991) The medium chains of the mammalian clathrin-associated proteins have a homolog in yeast. Eur J Biochem 202(2):569-74
2)	Stepp JD, et al. (1995) A late Golgi sorting function for Saccharomyces cerevisiae Apm1p, but not for Apm2p, a second yeast clathrin AP medium chain-related protein. Mol Biol Cell 6(1):41-58
3)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41