ENV7/YPL236C Protein Information

Standard Name	Env7p 1
Systematic Name	Ypl236cp
ORF Classification	Verified
Description	Vacuolar membrane protein kinase; negatively regulates membrane fusion; associates with vacuolar membrane through palmitoylation of one or more cysteines in consensus sequence; vacuolar membrane association is essential to its kinase activity; mutant shows defect in CPY processing; ortholog of human serine/threonine kinase 16 (STK16) (1, 2)
Name Description	late ENdosome and Vacuole interface function 1

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	364
Molecular Weight (Da)	40,748
Isoelectric Point (pI)	4.88

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Env7p (InterPro)
Physical Interactions	There are 9 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI000006B12E \| Q12003 MIPS: YPL236C NCBI: 1061253 \| 1370488 \| 2326841 \| 6325020 \| 74676496 \| NP_015088.1 GenBank/EMBL/DDBJ: DAA11200.1 \| Z67751 \| Z73591 \| Z73592
External Classifications	EC: 2.7.11.1 [Non-specific serine/threonine protein kinase]

Amino Acid Sequence (or in FASTA format)
1 MISIVLELFQ NLCCCRGFSD ATIRVNDKRY RIQRLLGEGG MSFVYLVQLS 51 KNSLIIDNGI ATPELYALKK IICPSVESIS NGMREIENYK RFQSPYVIKS 101 IDSQVMQEKD GSKTIYIVLP YYSLGSLQDS INRRLLEGTF VSEAECVRIM 151 LGVTRGLLCL HDPASRQDNA TSRVNVDAVS MTYSDETAML LEDTPLEMDM 201 LSSNSAGSIA YAHRDITPSN ILFSSDGLPV IGDLGSCSQA DITIENRHQL 251 SELQEWVNDN CTLPYTPPEL LNLKLNQVLS SKVDIWSLGC TFYTLMFGIS 301 PFEREEQIHG ASLTYAINTG KYSFPRNSRF SEGLLSVIKK CIQVDPIQRP 351 TTSQLLNLLQ DLDT*

Amino Acid Sequence (or in FASTA format)

       1  MISIVLELFQ NLCCCRGFSD ATIRVNDKRY RIQRLLGEGG MSFVYLVQLS
      51  KNSLIIDNGI ATPELYALKK IICPSVESIS NGMREIENYK RFQSPYVIKS
     101  IDSQVMQEKD GSKTIYIVLP YYSLGSLQDS INRRLLEGTF VSEAECVRIM
     151  LGVTRGLLCL HDPASRQDNA TSRVNVDAVS MTYSDETAML LEDTPLEMDM
     201  LSSNSAGSIA YAHRDITPSN ILFSSDGLPV IGDLGSCSQA DITIENRHQL
     251  SELQEWVNDN CTLPYTPPEL LNLKLNQVLS SKVDIWSLGC TFYTLMFGIS
     301  PFEREEQIHG ASLTYAINTG KYSFPRNSRF SEGLLSVIKK CIQVDPIQRP
     351  TTSQLLNLLQ DLDT*

external links for Env7p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	Organelle DB
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YPL+
Aspergillus (AspGD)	BioPIXIE	MIPS	YeastGFP
Candida (CGD)	CYC2008 (complexes)	Pfam domains	YeastRC Public Image Repository
Candida (CandidaDB)	Complexome	YeastRC Structure Prediction (Seattle)
YGOB	DIP
YOGY	GeneMANIA
	YeastRC Two-Hybrid (Seattle)

References cited on this page View Complete Literature Guide for Env7p

1)	Ricarte F, et al. (2011) A Genome-Wide Immunodetection Screen in S. cerevisiae Uncovers Novel Genes Involved in Lysosomal Vacuole Function and Morphology. PLoS One 6(8):e23696
2)	Manandhar SP, et al. (2013) Saccharomyces cerevisiae Env7 is a novel serine/threonine kinase 16-related protein kinase and negatively regulates organelle fusion at the lysosomal vacuole. Mol Cell Biol 33(3):526-42